209-401-B99

antibody from Rockland Immunochemicals, Inc.

Targeting: VEGFA VEGF, VEGF-A, VPF

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

209-401-B99

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#209-401-B99, RRID:AB_10707145

Product name

Anti-VEGF-165 (RABBIT) Antibody - 209-401-B99

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Rockland's anti-Human VEGF-165 antibody shows detection of a band ~22 kDa in size corresponding to recombinant human VEGF-165. This isoform of VEGF tends to form a disulfide linked homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).

Validation comment

Western Blot