Antibody data
- Antibody Data
- Antigen structure
- References [60]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
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- Product number
- MA5-12184 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VEGF Monoclonal Antibody (VG1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-12184 targets Vascular Endothelial Growth Factor in IF, IHC (P) and WB applications and shows reactivity with Human samples.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- VG1
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references Proteoglycan 4 (PRG4) treatment enhances wound closure and tissue regeneration.
Identifying Common Pathogenic Features in Deep Endometriotic Nodules and Uterine Adenomyosis.
Macrophage migration inhibitory factor inhibition as a novel therapeutic approach against triple-negative breast cancer.
Effects of formaldehyde on vascular endothelial growth factor, matrix metallopeptidase 2 and osteonectin levels in periodontal membrane and alveolar bone in rats.
A lasered mouse model of retinal degeneration displays progressive outer retinal pathology providing insights into early geographic atrophy.
Squalamine blocks tumor-associated angiogenesis and growth of human breast cancer cells with or without HER-2/neu overexpression.
Inflammatory-Driven Angiogenesis in Bone Augmentation with Bovine Hydroxyapatite, B-Tricalcium Phosphate, and Bioglasses: A Comparative Study.
Immunoexpression of vascular endothelial growth factor and B-cell lymphoma 2 in the uterine tissue of rats treated with melatonin in the estrus phase1.
The Eyes Absent Proteins in Developmental and Pathological Angiogenesis.
Coronary thrombi neovascularization in patients with ST-elevation myocardial infarction - clinical and angiographic implications.
Thymic epithelial tumors express vascular endothelial growth factors and their receptors as potential targets of antiangiogenic therapy: a tissue micro array-based multicenter study.
Oxygen-induced retinopathy in mice with retinal photoreceptor cell degeneration.
Mesenchymal-to-endothelial transition in Kaposi sarcoma: a histogenetic hypothesis based on a case series and literature review.
Resveratrol treatment delays growth plate fusion and improves bone growth in female rabbits.
Temporal and pharmacological characterization of angiostatin release and generation by human platelets: implications for endothelial cell migration.
Diagnostic and therapeutic strategy and the most efficient prognostic factors of breast malignant fibrous histiocytoma.
Biological and demographic profile of meningiomas in a cohort of Egyptian patients: impact on tumor recurrence.
Paraneoplastic symptoms: cachexia, polycythemia, and hypercalcemia are, respectively, related to vascular endothelial growth factor (VEGF) expression in renal clear cell carcinoma.
The relevance of the intrinsic subtype to the clinicopathological features and biomarkers in Japanese breast cancer patients.
The impact of KRAS mutations on VEGF-A production and tumour vascular network.
The effect of lipoic acid on wound healing in a full thickness uterine injury model in rats.
The efficacy of bevacizumab, sorafenib, and retinoic acid on rat endometriosis model.
Paraneoplastic hormones: parathyroid hormone-related protein (PTHrP) and erythropoietin (EPO) are related to vascular endothelial growth factor (VEGF) expression in clear cell renal cell carcinoma.
Effects of methylene blue, pentoxyphylline and enoxaparin on postoperative adhesion formation and markers of angiogenesis in a rat uterine horn model.
Pulmonary artery sarcoma with angiosarcoma phenotype mimicking pleomorphic malignant fibrous histiocytoma: a case report.
Boswellic acid inhibits growth and metastasis of human colorectal cancer in orthotopic mouse model by downregulating inflammatory, proliferative, invasive and angiogenic biomarkers.
Lipoxin A4 inhibits the development of endometriosis in mice: the role of anti-inflammation and anti-angiogenesis.
Bronchial carcinoid tumors metastatic to the sella turcica and review of the literature.
Immunohistochemical study of the angiogenetic network of VEGF, HIF1α, VEGFR-2 and endothelial nitric oxide synthase (eNOS) in human breast cancer.
Prediction of the shrinking rate of uterine leiomyoma nodules using needle biopsy specimens.
Identification of prognostic biomarkers for glioblastomas using protein expression profiling.
The role of thrombin receptors PAR1 and PAR4 for PAI-1 storage, synthesis and secretion by human platelets.
Immunolocalization of VEGF, VEGF receptors, EGF-R and Ki-67 in leiomyoma, cellular leiomyoma and leiomyosarcoma.
Angiogenesis in the progression of breast ductal proliferations.
VEGFR1 expression is related to lymph node metastasis and serum VEGF may be a marker of progression in the follow-up of patients with differentiated thyroid carcinoma.
Serum and pulmonary vascular endothelial growth factor/receptors and haemodynamic measurements in cyanotic congenital heart disease with decreased pulmonary blood flow.
Prevention of ovarian hyperstimulation syndrome in a rat model: efficacy comparison between cabergoline and meloxicam.
Evaluation of relationship between HIF-1alpha immunoreactivity and stage, grade, angiogenic profile and proliferative index in bladder urothelial carcinomas.
Molecular biomarkers for advanced renal cell carcinoma: implications for prognosis and therapy.
Podocyte-specific overexpression of GLUT1 surprisingly reduces mesangial matrix expansion in diabetic nephropathy in mice.
Anti-human vascular endothelial growth factor (VEGF) antibody selection for immunohistochemical staining of proliferating blood vessels.
Maspin, VEGF and p53 expression in small biopsies of primary advanced lung cancer and relationship with clinicopathologic parameters.
Vascular endothelial growth factor: a possible mediator of endothelial activation in acute respiratory distress syndrome.
The differences between the endothelial area marked with CD31 and CD105 in colorectal carcinomas by computer-assisted morphometrical analysis.
Clinical and immunohistochemical features associated with a response to bortezomib in patients with multiple myeloma.
Bevacizumab and breast cancer: current therapeutic progress and future perspectives.
The expression of cytoskeleton regulatory protein Mena in colorectal lesions.
Association of vascular endothelial growth factor and vascular endothelial growth factor receptor-2 genetic polymorphisms with outcome in a trial of paclitaxel compared with paclitaxel plus bevacizumab in advanced breast cancer: ECOG 2100.
Neoangiogenesis and expression of hypoxia-inducible factor 1alpha, vascular endothelial growth factor, and glucose transporter-1 in endometrioid type endometrium adenocarcinomas.
Vascular endothelial growth factor expression and vascularity in renal allograft biopsies.
Vascular endothelial growth factor, hypoxia-inducible factor 1 alpha and CD34 expressions in early-stage gastric tumors: relationship with pathological factors and prognostic impact on survival.
Evaluation of the prognostic role of a panel of biomarkers in stage IB-IIIA non-small cell lung cancer patients.
Immunohistochemical analysis with multiple antibodies in search of prognostic markers for clear cell renal cell carcinoma.
Angiogenesis in nodal B cell lymphomas: a high throughput study.
Prognostic significance of HIF-2alpha/EPAS1 expression in hepatocellular carcinoma.
Prognostic significance of HIF-2alpha/EPAS1 expression in hepatocellular carcinoma.
Effects of stobadine and vitamin E in diabetes-induced retinal abnormalities: involvement of oxidative stress.
Effects of finasteride on the vascular surface density, number of microvessels and vascular endothelial growth factor expression of the rat prostate.
Microvessel density, VEGF expression, and tumor-associated macrophages in breast tumors: correlations with prognostic parameters.
PGE1 analog alprostadil induces VEGF and eNOS expression in endothelial cells.
Krawetz RJ, Abubacker S, Leonard C, Masson AO, Shah S, Narendran N, Tailor P, Regmi SC, Labit E, Ninkovic N, Corpuz JM, Ito K, Underhill TM, Salo PT, Schmidt TA, Biernaskie JA
NPJ Regenerative medicine 2022 Jun 24;7(1):32
NPJ Regenerative medicine 2022 Jun 24;7(1):32
Identifying Common Pathogenic Features in Deep Endometriotic Nodules and Uterine Adenomyosis.
Stratopoulou CA, Camboni A, Donnez J, Dolmans MM
Journal of clinical medicine 2021 Oct 4;10(19)
Journal of clinical medicine 2021 Oct 4;10(19)
Macrophage migration inhibitory factor inhibition as a novel therapeutic approach against triple-negative breast cancer.
Charan M, Das S, Mishra S, Chatterjee N, Varikuti S, Kaul K, Misri S, Ahirwar DK, Satoskar AR, Ganju RK
Cell death & disease 2020 Sep 17;11(9):774
Cell death & disease 2020 Sep 17;11(9):774
Effects of formaldehyde on vascular endothelial growth factor, matrix metallopeptidase 2 and osteonectin levels in periodontal membrane and alveolar bone in rats.
Laçin N, İzol BS, Tuncer MC, Gökalp Özkorkmaz E, Deveci B, Deveci E
Folia morphologica 2019;78(3):545-553
Folia morphologica 2019;78(3):545-553
A lasered mouse model of retinal degeneration displays progressive outer retinal pathology providing insights into early geographic atrophy.
Ibbett P, Goverdhan SV, Pipi E, Chouhan JK, Keeling E, Angus EM, Scott JA, Gatherer M, Page A, Teeling JL, Lotery AJ, Arjuna Ratnayaka J
Scientific reports 2019 May 16;9(1):7475
Scientific reports 2019 May 16;9(1):7475
Squalamine blocks tumor-associated angiogenesis and growth of human breast cancer cells with or without HER-2/neu overexpression.
Márquez-Garbán DC, Gorrín-Rivas M, Chen HW, Sterling C Jr, Elashoff D, Hamilton N, Pietras RJ
Cancer letters 2019 May 1;449:66-75
Cancer letters 2019 May 1;449:66-75
Inflammatory-Driven Angiogenesis in Bone Augmentation with Bovine Hydroxyapatite, B-Tricalcium Phosphate, and Bioglasses: A Comparative Study.
Anghelescu VM, Neculae I, Dincă O, Vlădan C, Socoliuc C, Cioplea M, Nichita L, Popp C, Zurac S, Bucur A
Journal of immunology research 2018;2018:9349207
Journal of immunology research 2018;2018:9349207
Immunoexpression of vascular endothelial growth factor and B-cell lymphoma 2 in the uterine tissue of rats treated with melatonin in the estrus phase1.
Görük NY, Deveci E
Acta cirurgica brasileira 2018 Jul;33(7):629-640
Acta cirurgica brasileira 2018 Jul;33(7):629-640
The Eyes Absent Proteins in Developmental and Pathological Angiogenesis.
Wang Y, Tadjuidje E, Pandey RN, Stefater JA 3rd, Smith LE, Lang RA, Hegde RS
The American journal of pathology 2016 Mar;186(3):568-78
The American journal of pathology 2016 Mar;186(3):568-78
Coronary thrombi neovascularization in patients with ST-elevation myocardial infarction - clinical and angiographic implications.
Kostić J, Orlić D, Borović ML, Beleslin B, Milašinović D, Dobrić M, Tešić M, Ostojić M
Thrombosis research 2014 Nov;134(5):1038-45
Thrombosis research 2014 Nov;134(5):1038-45
Thymic epithelial tumors express vascular endothelial growth factors and their receptors as potential targets of antiangiogenic therapy: a tissue micro array-based multicenter study.
Lattanzio R, La Sorda R, Facciolo F, Sioletic S, Lauriola L, Martucci R, Gallo E, Palmieri G, Evoli A, Alessandrini G, Ruco L, Rendina EA, Truini M, Chiarle R, Barreca A, Pich A, Ascani S, Remotti D, Tunesi G, Granone P, Ratto GB, Puma F, Pescarmona E, Piantelli M, Marino M, Thymic Epithelial Tumor Working Group, Carlini S, Cerasoli V, Corzani F, Melis E, Filippetti M, Canalini P, Palestro G, Lalle M, Ruffini E, Ceribelli A, Rinaldi M
Lung cancer (Amsterdam, Netherlands) 2014 Aug;85(2):191-6
Lung cancer (Amsterdam, Netherlands) 2014 Aug;85(2):191-6
Oxygen-induced retinopathy in mice with retinal photoreceptor cell degeneration.
Zhang Q, Zhang ZM
Life sciences 2014 Apr 25;102(1):28-35
Life sciences 2014 Apr 25;102(1):28-35
Mesenchymal-to-endothelial transition in Kaposi sarcoma: a histogenetic hypothesis based on a case series and literature review.
Gurzu S, Ciortea D, Munteanu T, Kezdi-Zaharia I, Jung I
PloS one 2013;8(8):e71530
PloS one 2013;8(8):e71530
Resveratrol treatment delays growth plate fusion and improves bone growth in female rabbits.
Karimian E, Tamm C, Chagin AS, Samuelsson K, Kjartansdóttir KR, Ohlsson C, Sävendahl L
PloS one 2013;8(6):e67859
PloS one 2013;8(6):e67859
Temporal and pharmacological characterization of angiostatin release and generation by human platelets: implications for endothelial cell migration.
Radziwon-Balicka A, Moncada de la Rosa C, Zielnik B, Doroszko A, Jurasz P
PloS one 2013;8(3):e59281
PloS one 2013;8(3):e59281
Diagnostic and therapeutic strategy and the most efficient prognostic factors of breast malignant fibrous histiocytoma.
Qiu SQ, Wei XL, Huang WH, Wu MY, Qin YS, Li YK, Zhang GJ
Scientific reports 2013;3:2529
Scientific reports 2013;3:2529
Biological and demographic profile of meningiomas in a cohort of Egyptian patients: impact on tumor recurrence.
Abdelzaher E, El Deeb NM, Gowil AG, Yehya A
TheScientificWorldJournal 2013;2013:375139
TheScientificWorldJournal 2013;2013:375139
Paraneoplastic symptoms: cachexia, polycythemia, and hypercalcemia are, respectively, related to vascular endothelial growth factor (VEGF) expression in renal clear cell carcinoma.
Ding GX, Feng CC, Song NH, Fang ZJ, Xia GW, Jiang HW, Hua LX, Ding Q
Urologic oncology 2013 Nov;31(8):1820-5
Urologic oncology 2013 Nov;31(8):1820-5
The relevance of the intrinsic subtype to the clinicopathological features and biomarkers in Japanese breast cancer patients.
Tamaki M, Kamio T, Kameoka S, Kojimahara N, Nishikawa T
World journal of surgical oncology 2013 Nov 18;11:293
World journal of surgical oncology 2013 Nov 18;11:293
The impact of KRAS mutations on VEGF-A production and tumour vascular network.
Figueras A, Arbos MA, Quiles MT, Viñals F, Germà JR, Capellà G
BMC cancer 2013 Mar 18;13:125
BMC cancer 2013 Mar 18;13:125
The effect of lipoic acid on wound healing in a full thickness uterine injury model in rats.
Micili SC, Goker A, Sayin O, Akokay P, Ergur BU
Journal of molecular histology 2013 Jun;44(3):339-45
Journal of molecular histology 2013 Jun;44(3):339-45
The efficacy of bevacizumab, sorafenib, and retinoic acid on rat endometriosis model.
Ozer H, Boztosun A, Açmaz G, Atilgan R, Akkar OB, Kosar MI
Reproductive sciences (Thousand Oaks, Calif.) 2013 Jan;20(1):26-32
Reproductive sciences (Thousand Oaks, Calif.) 2013 Jan;20(1):26-32
Paraneoplastic hormones: parathyroid hormone-related protein (PTHrP) and erythropoietin (EPO) are related to vascular endothelial growth factor (VEGF) expression in clear cell renal cell carcinoma.
Feng CC, Ding GX, Song NH, Li X, Wu Z, Jiang HW, Ding Q
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2013 Dec;34(6):3471-6
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2013 Dec;34(6):3471-6
Effects of methylene blue, pentoxyphylline and enoxaparin on postoperative adhesion formation and markers of angiogenesis in a rat uterine horn model.
Boztosun A, Piçnak A, Kosar MI, Gulturk S, Cetin A
Clinical and experimental obstetrics & gynecology 2012;39(1):89-95
Clinical and experimental obstetrics & gynecology 2012;39(1):89-95
Pulmonary artery sarcoma with angiosarcoma phenotype mimicking pleomorphic malignant fibrous histiocytoma: a case report.
Bohn OL, de León EA, Lezama O, Rios-Luna NP, Sánchez-Sosa S, Llombart-Bosch A
Diagnostic pathology 2012 Nov 7;7:154
Diagnostic pathology 2012 Nov 7;7:154
Boswellic acid inhibits growth and metastasis of human colorectal cancer in orthotopic mouse model by downregulating inflammatory, proliferative, invasive and angiogenic biomarkers.
Yadav VR, Prasad S, Sung B, Gelovani JG, Guha S, Krishnan S, Aggarwal BB
International journal of cancer 2012 May 1;130(9):2176-84
International journal of cancer 2012 May 1;130(9):2176-84
Lipoxin A4 inhibits the development of endometriosis in mice: the role of anti-inflammation and anti-angiogenesis.
Xu Z, Zhao F, Lin F, Chen J, Huang Y
American journal of reproductive immunology (New York, N.Y. : 1989) 2012 Jun;67(6):491-7
American journal of reproductive immunology (New York, N.Y. : 1989) 2012 Jun;67(6):491-7
Bronchial carcinoid tumors metastatic to the sella turcica and review of the literature.
Moshkin O, Rotondo F, Scheithauer BW, Soares M, Coire C, Smyth HS, Goth M, Horvath E, Kovacs K
Pituitary 2012 Jun;15(2):160-5
Pituitary 2012 Jun;15(2):160-5
Immunohistochemical study of the angiogenetic network of VEGF, HIF1α, VEGFR-2 and endothelial nitric oxide synthase (eNOS) in human breast cancer.
Kafousi M, Vrekoussis T, Tsentelierou E, Pavlakis K, Navrozoglou I, Dousias V, Sanidas E, Tsiftsis D, Georgoulias V, Stathopoulos EN
Pathology oncology research : POR 2012 Jan;18(1):33-41
Pathology oncology research : POR 2012 Jan;18(1):33-41
Prediction of the shrinking rate of uterine leiomyoma nodules using needle biopsy specimens.
Kasai M, Ichimura T, Kawamura N, Sumikura T, Matsuda M, Asano C, Sumi T, Ishiko O
Fertility and sterility 2012 Aug;98(2):440-3
Fertility and sterility 2012 Aug;98(2):440-3
Identification of prognostic biomarkers for glioblastomas using protein expression profiling.
Jung Y, Joo KM, Seong DH, Choi YL, Kong DS, Kim Y, Kim MH, Jin J, Suh YL, Seol HJ, Shin CS, Lee JI, Kim JH, Song SY, Nam DH
International journal of oncology 2012 Apr;40(4):1122-32
International journal of oncology 2012 Apr;40(4):1122-32
The role of thrombin receptors PAR1 and PAR4 for PAI-1 storage, synthesis and secretion by human platelets.
Nylander M, Osman A, Ramström S, Aklint E, Larsson A, Lindahl TL
Thrombosis research 2012 Apr;129(4):e51-8
Thrombosis research 2012 Apr;129(4):e51-8
Immunolocalization of VEGF, VEGF receptors, EGF-R and Ki-67 in leiomyoma, cellular leiomyoma and leiomyosarcoma.
Sanci M, Dikis C, Inan S, Turkoz E, Dicle N, Ispahi C
Acta histochemica 2011 May;113(3):317-25
Acta histochemica 2011 May;113(3):317-25
Angiogenesis in the progression of breast ductal proliferations.
Carpenter PM, Chen WP, Mendez A, McLaren CE, Su MY
International journal of surgical pathology 2011 Jun;19(3):335-41
International journal of surgical pathology 2011 Jun;19(3):335-41
VEGFR1 expression is related to lymph node metastasis and serum VEGF may be a marker of progression in the follow-up of patients with differentiated thyroid carcinoma.
Karaca Z, Tanriverdi F, Unluhizarci K, Ozturk F, Gokahmetoglu S, Elbuken G, Cakir I, Bayram F, Kelestimur F
European journal of endocrinology 2011 Feb;164(2):277-84
European journal of endocrinology 2011 Feb;164(2):277-84
Serum and pulmonary vascular endothelial growth factor/receptors and haemodynamic measurements in cyanotic congenital heart disease with decreased pulmonary blood flow.
Tolunay I, Tunaoglu S, Akyürek N, Halid V, Olgunturk R, Kula S
Cardiology in the young 2011 Dec;21(6):608-15
Cardiology in the young 2011 Dec;21(6):608-15
Prevention of ovarian hyperstimulation syndrome in a rat model: efficacy comparison between cabergoline and meloxicam.
Saylan A, Arioz DT, Koken T, Dilek H, Saylan F, Yilmazer M
Acta obstetricia et gynecologica Scandinavica 2010 May;89(5):692-9
Acta obstetricia et gynecologica Scandinavica 2010 May;89(5):692-9
Evaluation of relationship between HIF-1alpha immunoreactivity and stage, grade, angiogenic profile and proliferative index in bladder urothelial carcinomas.
Deniz H, Karakök M, Yagci F, Güldür ME
International urology and nephrology 2010 Mar;42(1):103-7
International urology and nephrology 2010 Mar;42(1):103-7
Molecular biomarkers for advanced renal cell carcinoma: implications for prognosis and therapy.
Kim HS, Kim WS, Park SH, Jung CW, Choi HY, Lee HM, Jeon SS, Ha H, Hwang IG, Lee S, Lim HY
Urologic oncology 2010 Mar-Apr;28(2):157-63
Urologic oncology 2010 Mar-Apr;28(2):157-63
Podocyte-specific overexpression of GLUT1 surprisingly reduces mesangial matrix expansion in diabetic nephropathy in mice.
Zhang H, Schin M, Saha J, Burke K, Holzman LB, Filipiak W, Saunders T, Xiang M, Heilig CW, Brosius FC 3rd
American journal of physiology. Renal physiology 2010 Jul;299(1):F91-8
American journal of physiology. Renal physiology 2010 Jul;299(1):F91-8
Anti-human vascular endothelial growth factor (VEGF) antibody selection for immunohistochemical staining of proliferating blood vessels.
van der Loos CM, Meijer-Jorna LB, Broekmans ME, Ploegmakers HP, Teeling P, de Boer OJ, van der Wal AC
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2010 Feb;58(2):109-18
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2010 Feb;58(2):109-18
Maspin, VEGF and p53 expression in small biopsies of primary advanced lung cancer and relationship with clinicopathologic parameters.
Bircan A, Bircan S, Kapucuoglu N, Songur N, Ozturk O, Akkaya A
Pathology oncology research : POR 2010 Dec;16(4):553-61
Pathology oncology research : POR 2010 Dec;16(4):553-61
Vascular endothelial growth factor: a possible mediator of endothelial activation in acute respiratory distress syndrome.
Azamfirei L, Gurzu S, Solomon R, Copotoiu R, Copotoiu S, Jung I, Tilinca M, Branzaniuc K, Corneci D, Szederjesi J, Kovacs J
Minerva anestesiologica 2010 Aug;76(8):609-16
Minerva anestesiologica 2010 Aug;76(8):609-16
The differences between the endothelial area marked with CD31 and CD105 in colorectal carcinomas by computer-assisted morphometrical analysis.
Jung I, Gurzu S, Raica M, Cîmpean AM, Szentirmay Z
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2009;50(2):239-43
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2009;50(2):239-43
Clinical and immunohistochemical features associated with a response to bortezomib in patients with multiple myeloma.
Dawson MA, Opat SS, Taouk Y, Donovan M, Zammit M, Monaghan K, Horvath N, Roberts AW, Prince HM, Hertzberg M, McLean CA, Spencer A
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 15;15(2):714-22
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 15;15(2):714-22
Bevacizumab and breast cancer: current therapeutic progress and future perspectives.
Yang SX
Expert review of anticancer therapy 2009 Dec;9(12):1715-25
Expert review of anticancer therapy 2009 Dec;9(12):1715-25
The expression of cytoskeleton regulatory protein Mena in colorectal lesions.
Gurzu S, Jung I, Prantner I, Ember I, Pávai Z, Mezei T
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2008;49(3):345-9
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2008;49(3):345-9
Association of vascular endothelial growth factor and vascular endothelial growth factor receptor-2 genetic polymorphisms with outcome in a trial of paclitaxel compared with paclitaxel plus bevacizumab in advanced breast cancer: ECOG 2100.
Schneider BP, Wang M, Radovich M, Sledge GW, Badve S, Thor A, Flockhart DA, Hancock B, Davidson N, Gralow J, Dickler M, Perez EA, Cobleigh M, Shenkier T, Edgerton S, Miller KD, ECOG 2100
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2008 Oct 1;26(28):4672-8
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2008 Oct 1;26(28):4672-8
Neoangiogenesis and expression of hypoxia-inducible factor 1alpha, vascular endothelial growth factor, and glucose transporter-1 in endometrioid type endometrium adenocarcinomas.
Ozbudak IH, Karaveli S, Simsek T, Erdogan G, Pestereli E
Gynecologic oncology 2008 Mar;108(3):603-8
Gynecologic oncology 2008 Mar;108(3):603-8
Vascular endothelial growth factor expression and vascularity in renal allograft biopsies.
Sarioğlu S, Celik A, Erşen A, Uçer I, Sağlam F, Camsari T, Gülay H
Transplantation proceedings 2008 Jan-Feb;40(1):178-80
Transplantation proceedings 2008 Jan-Feb;40(1):178-80
Vascular endothelial growth factor, hypoxia-inducible factor 1 alpha and CD34 expressions in early-stage gastric tumors: relationship with pathological factors and prognostic impact on survival.
Cabuk D, Basaran G, Celikel C, Dane F, Yumuk PF, Iyikesici MS, Ekenel M, Turhal NS
Oncology 2007;72(1-2):111-7
Oncology 2007;72(1-2):111-7
Evaluation of the prognostic role of a panel of biomarkers in stage IB-IIIA non-small cell lung cancer patients.
Timotheadou E, Skarlos DV, Samantas E, Papadopoulos S, Murray S, Skrickova J, Christodoulou C, Papakostantinou C, Pectasides D, Papakostas P, Kaplanova J, Vrettou E, Karina M, Kosmidis P, Fountzilas G
Anticancer research 2007 Nov-Dec;27(6C):4481-9
Anticancer research 2007 Nov-Dec;27(6C):4481-9
Immunohistochemical analysis with multiple antibodies in search of prognostic markers for clear cell renal cell carcinoma.
Phuoc NB, Ehara H, Gotoh T, Nakano M, Yokoi S, Deguchi T, Hirose Y
Urology 2007 May;69(5):843-8
Urology 2007 May;69(5):843-8
Angiogenesis in nodal B cell lymphomas: a high throughput study.
Tzankov A, Heiss S, Ebner S, Sterlacci W, Schaefer G, Augustin F, Fiegl M, Dirnhofer S
Journal of clinical pathology 2007 May;60(5):476-82
Journal of clinical pathology 2007 May;60(5):476-82
Prognostic significance of HIF-2alpha/EPAS1 expression in hepatocellular carcinoma.
Bangoura G, Liu ZS, Qian Q, Jiang CQ, Yang GF, Jing S
World journal of gastroenterology 2007 Jun 21;13(23):3176-82
World journal of gastroenterology 2007 Jun 21;13(23):3176-82
Prognostic significance of HIF-2alpha/EPAS1 expression in hepatocellular carcinoma.
Bangoura G, Liu ZS, Qian Q, Jiang CQ, Yang GF, Jing S
World journal of gastroenterology 2007 Jun 21;13(23):3176-82
World journal of gastroenterology 2007 Jun 21;13(23):3176-82
Effects of stobadine and vitamin E in diabetes-induced retinal abnormalities: involvement of oxidative stress.
Yülek F, Or M, Ozoğul C, Isik AC, Ari N, Stefek M, Bauer V, Karasu C
Archives of medical research 2007 Jul;38(5):503-11
Archives of medical research 2007 Jul;38(5):503-11
Effects of finasteride on the vascular surface density, number of microvessels and vascular endothelial growth factor expression of the rat prostate.
Canda AE, Mungan MU, Yilmaz O, Yorukoglu K, Tuzel E, Kirkali Z
International urology and nephrology 2006;38(2):275-80
International urology and nephrology 2006;38(2):275-80
Microvessel density, VEGF expression, and tumor-associated macrophages in breast tumors: correlations with prognostic parameters.
Bolat F, Kayaselcuk F, Nursal TZ, Yagmurdur MC, Bal N, Demirhan B
Journal of experimental & clinical cancer research : CR 2006 Sep;25(3):365-72
Journal of experimental & clinical cancer research : CR 2006 Sep;25(3):365-72
PGE1 analog alprostadil induces VEGF and eNOS expression in endothelial cells.
Haider DG, Bucek RA, Giurgea AG, Maurer G, Glogar H, Minar E, Wolzt M, Mehrabi MR, Baghestanian M
American journal of physiology. Heart and circulatory physiology 2005 Nov;289(5):H2066-72
American journal of physiology. Heart and circulatory physiology 2005 Nov;289(5):H2066-72
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Vascular Endothelial Growth Factor using Vascular Endothelial Growth Factor Monoclonal Antibody (Product # MA5-12184) on VEGF Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Vascular Endothelial Growth Factor (VEGF) was performed by loading 1 µg of indicated recombinant VEGF isoforms, and 10 µL of PageRuler PlusPrestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane (Product # 88518) using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. VEGF was detected at 14 kD, 19 kD and 22-30 kD using a VEGF monoclonal antibody (Product # MA5-12184) at a concentration of 1 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody (Product # 31437) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076).
- Submitted by
- Invitrogen Antibodies (provider)
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- Western blot was performed using Anti-VEGF Monoclonal Antibody (VG1) (Product # MA5-12184) and a 52 kDa band corresponding to VEGF was observed across cell line tested in Figure a and 20 and 40 kDa bands in recombinant VEGF protein in Figure b. Whole cell extracts (30 µg lysate) of HACAT cells and HACAT cells treated with 0.5 mM H2O2 for 30 minutes followed by 1X-PTI for 4 hours in Figure a and recombinant VEGF protein (250 ng and 25 ng) in Figure b were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).
Supportive validation
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- Immunofluorescence analysis of VEGF was performed using DU 145 cells and DU 145 cells treated with 1X-PTI for 4 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with VEGF Monoclonal Antibody (VG1) (Product # MA5-12184) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of DU 145 PTI-treated cells showing induction of VEGF protein in cytoplasm (golgi apparatus). Panel e represents the merged image of untreated DU 145 cells, that shows lower expression for VEGF protein. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
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- Invitrogen Antibodies (provider)
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- Immunofluorescence analysis of VEGF was performed using HACAT cells and HACAT cells treated with 0.5 mM H2O2 for 30 minutes followed by 1X-PTI for 4 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with VEGF Monoclonal Antibody (VG1) (Product # MA5-12184) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing induction of VEGF expression in cytoplasm in PTI-treated HACAT cells. Panel e represents untreated HACAT cells showing lower VEGF expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
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- Immunofluorescent analysis of Vascular Endothelial Growth Factor (VEGF, green) in Hela cells. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with (left panel) or without (right panel) a VEGF polyclonal antibody (Product # MA5-12184) at a dilution of 1:40 for at least 1 hour at room temperature, and then incubated with a Dylight 488 goat anti-Mouse IgG secondary antibody at a dilution of 1:1000 for 45 minutes at room temperature. F-actin (both panels, red) was stained by Dylight 554 Phalloidin (Product # 21834) and nuclei (both panels, blue) were stained with DAPI (Product # 46190). Images were taken at 60X magnification.
Supportive validation
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- Formalin-fixed, paraffin-embedded human angiosarcoma stained with VEGF antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.
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- Figure 3 Representative images of VEGF expression, MVD, and presence of alphaSMA+ cells around vessels in endometrium: healthy; DEN eutopic; DEN lesion; AD eutopic; AD lesion. Scale bar (in black) represents 100 mum.