Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-36369 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JIP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 147 kDa.
- Concentration
- 1 mg/mL
Submitted references Dual Leucine Zipper Kinase Is Constitutively Active in the Adult Mouse Brain and Has Both Stress-Induced and Homeostatic Functions.
Goodwani S, Fernandez C, Acton PJ, Buggia-Prevot V, McReynolds ML, Ma J, Hu CH, Hamby ME, Jiang Y, Le K, Soth MJ, Jones P, Ray WJ
International journal of molecular sciences 2020 Jul 9;21(14)
International journal of molecular sciences 2020 Jul 9;21(14)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of JIP-3 using JIP-3 polyclonal antibody (Product # PA5-36369) at a dilution of 1:500. Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: PC12 whole cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of JIP3 in Lane 1: L02 whole cell lysate (40 µg), Lane 2: HEK293T whole cell lysate (40 µg), Lane 3: HT102 whole cell lysate (40 µg). Samples were incubated with JIP3 polyclonal antibody (Product # PA5-36369) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of JIP-3 in paraffin-embedded human breast carcinoma using JIP-3 polyclonal antibody (Product # PA5-36369) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 DLK is constitutively active in all the brain regions of adult mouse brain but has brain region specific scaffolding proteins. ( a ) Representative immunoblots of DLK from the homogenates obtained from nTg and Tg forebrain of 2, 4, 6, and 8 months old female mice. ( b ) Quantification of the immunoblots of DLK/GAPDH from the nTg and Tg forebrain homogenates from 2, 4, 6, and 8 months old female mice, plotted as mean fold change relative to nTg group of respective age cohort. Unpaired t -test. + p < 0.05, ++ p < 0.01, n = 6. ( c ) Immunoblots showing differential expression of DLK signaling components: p-MKK4, MKK4, JNK1, JNK2, JNK3, p-JNK, JNK, p-c-Jun, and c-Jun from cerebellum (Cbm) and forebrain (Fore) from 3-4 months old male WT mice. ( d ) Representative immunoblots of p-MKK4 and MKK4 in homogenates from nTg cerebellum (nTg Cbm), nTg forebrain (nTg Fore), and Tg forebrain (Tg Fore) of 7-8 months old female mice treated with 50 mg/kg of DLKi or vehicle QD for 7 days. ( e ) Quantification of the immunoblots of p-MKK4/GAPDH and MKK4/GAPDH from homogenates obtained from nTg cerebellum, nTg forebrain, and Tg forebrain of mice treated with vehicle or DLKi, plotted as mean fold change relative to vehicle group in the respective brain region. Unpaired t -test. ** p < 0.01, n = 6. ( f ) Immunoblots showing differential expression of DLK scaffolding proteins: JIP1, JIP2, JIP3, and POSH from cerebellum (Cbm) and forebrain (Fore) from 3-4 months old male WT mice.