CABT-BL2332

antibody from Creative Diagnostics

Targeting: MBD1 CXXC3, PCM1

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

CABT-BL2332

Provider

Creative Diagnostics

Product name

Anti-MBD1 monoclonal antibody, clone 5G7

Antibody type

Monoclonal

Description

Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone Lys-9 trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

5G7

Storage

Store at -2°C or lower. Aliquot to avoid repeated freezing and thawing.