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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- H00004152-B01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004152-B01, RRID:AB_1114250
- Product name
- MBD1 MaxPab mouse polyclonal antibody (B01)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human MBD1 protein.
- Antigen sequence
MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYY
QSPTGDRIRSKVELTRYLGPACDLTLFDFKQGILC
YPAPKAHPVAVASKKRKKPSRPAKTRKRQVGPQSG
EVRKEAPRDETKADTDTAPASFPAPGCCENCGISF
SGDGTQRQRLKTLCKDCRAQRIAFNREQRMFKRVG
CGECAACQVTEDCGACSTCLLQLPHDVASGLFCKC
ERRRCLRIVERSRGCGVCRGCQTQEDCGHCPICLR
PPRPGLRRQWKCVQRRCLRGKHARRKGGCDSKMAA
RRRPGAQPLPPPPPSQSPEPTEPHPRALAPSPPAE
FIYYCVDEDELQPYTNRRQNRKCGACAACLRRMDC
GRCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRL
LPSVWSESEDGAGSPPPYRRRKRPSSARRHHLGPT
LKPTLATRTAQPDHTQAPTKQEAGGGFVLPPPGTD
LVFLREGASSPVQVPGPVAASTEALLQEAQCSGLS
WVVALPQVKQEKADTQDEWTPGTAVLTSPVLVPGC
PSKAVDPGLPSVKQEPPDPEEDKEENKDDSASKLA
PEEEAGGAGTPVITEIFSLGGTRFRDTAVWLPRSK
DLKKPGARKQ- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability.
The centriolar satellite protein Cep131 is important for genome stability.
Staples CJ, Myers KN, Beveridge RD, Patil AA, Howard AE, Barone G, Lee AJ, Swanton C, Howell M, Maslen S, Skehel JM, Boulton SJ, Collis SJ
Journal of cell science 2014 Jul 1;127(Pt 13):2910-9
Journal of cell science 2014 Jul 1;127(Pt 13):2910-9
The centriolar satellite protein Cep131 is important for genome stability.
Staples CJ, Myers KN, Beveridge RD, Patil AA, Lee AJ, Swanton C, Howell M, Boulton SJ, Collis SJ
Journal of cell science 2012 Oct 15;125(Pt 20):4770-9
Journal of cell science 2012 Oct 15;125(Pt 20):4770-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of MBD1 expression in transfected 293T cell line (H00004152-T01) by MBD1 MaxPab polyclonal antibody.Lane 1: MBD1 transfected lysate(66.55 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to MBD1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
