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LearnMA1-41025
antibody from Invitrogen Antibodies
Targeting: MBD1
CXXC3, PCM1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- MA1-41025 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MBD1 Monoclonal Antibody (100B272.1)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: Hela whole cell extract, HeLa cell lysate.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 100B272.1
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MBD1 in HeLa lysate using a MBD1 monoclonal antibody (Product # MA1-41025) at 2 µg/mL (lane 1) and 1 µg/mL (lane 2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MBD1 in HeLa lysate. Samples were incubated in MBD1 monoclonal antibody (Product # MA1-41025). Antibody concentration: 2 µg/mL (lane 1) and 1 µg/mL (lane 2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of MBD1 in NIH3T3 cells fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. Samples were incubated in MBD1 monoclonal antibody (Product # MA1-41025) using a dilution of 2 µg/mL overnight at 4 °C followed by anti-mouse DyLight 488 (Green) with a dilution of 1:500. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of MBD1 in HeLa cells fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. Samples were incubated in MBD1 monoclonal antibody (Product # MA1-41025) using a dilution of 2 µg/mL overnight at 4 °C followed by anti-mouse DyLight 488 (Green) with a dilution of 1:500. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MBD1 in formalin-fixed paraffin-embedded tissue section of human colon. Samples were incubated in MBD1 monoclonal antibody (Product # MA1-41025) using a dilution of 1:200 followed by HRP labeled anti-mouse secondary antibody and DAB reagent. Nuclei of cells were counter-stained with hematoxylin. Cytoplasmic and nuclear staining was observed.
