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LearnSTJ13100104
antibody from St John's Laboratory
Targeting: CLCN5
ClC-5, CLC5, DENTS, hCIC-K2, hClC-K2, NPHL1, NPHL2, XLRH, XRN
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [18]
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- Product number
- STJ13100104 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-CLCN5 antibody (STJ13100104)
- Antibody type
- Polyclonal
- Description
- Nz White Rabbit polyclonal antibody anti-CLCN5 is suitable for use in Immunohistochemistry and Western Blot research applications.
- Reactivity
- Human, Mouse, Rat
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat cerebellum. The section appears largely negative. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC-P on paraffin sections of rat olfactory bulb. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 100 ml of 37C PBS and then fixed with 300 ml 4% FA (37C) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0. 2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
