BS9277

antibody from Bioworld Technology, Inc

Targeting: TOP1

Western blot (Data presented on provider website)

Antibody Data

Product number

BS9277

Provider

Bioworld Technology, Inc

Product name

Topo I (W412) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide, corresponding to amino acids 381-430 of Human Topo I.

Description

DNA topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing both positive and negative supercoils, whereas prokaryotic topoisomerases relax only negative supercoils. DNA topoisomerases play a role in DNA replication, recombination and transcription and have been identified as targets of numerous anticancer drugs. Topo I, a ubiquitously expressed, soluble enzyme, acts by introducing a transient break in one strand of DNA, while Topo II acts by making a transient double-strand break. Topo II is encoded by two different genes to generate two distinct isoforms that are designated Topo IIÏ« and Topo IIÉÇ.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.