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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ARG55817 - Provider product page
- Provider
- Arigo
- Product name
- anti-Topoisomerase I antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein (N-terminus) of Human Topoisomerase I
- Description
- Purification with Protein G.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1291CT875.142.166
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of HUVEC, Jurkat, MCF-7, PC-12 cell line (from left to right) stained with ARG55817 anti-Topoisomerase I antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG55817 anti-Topoisomerase I antibody (green) at 1:25 dilution or isotype control antibody (blue), followed by incubation with Alexa Fluor? 488 labelled secondary antibody.
