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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- TA809934 - Provider product page
- Provider
- OriGene
- Product name
- TOP1 mouse monoclonal antibody,clone OTI3B9
- Antibody type
- Monoclonal
- Description
- TOP1 mouse monoclonal antibody,clone OTI3B9
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TOP1
- Isotype
- IgG
- Antibody clone number
- OTI3B9
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP1 (RC215658, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP1.(1:2000)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-TOP1 monoclonal antibody . (1:500)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809934)(1:500)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809934)(1:500)
- Validation comment
- IHC
