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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AM00154PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM00154PU-N, RRID:AB_1008144
- Product name
- anti TOP1 / SCL-70
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide conjugated to KLH.Epitope: amino acids 699-725.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 23B11
- Vial size
- 0.1 mg
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Detection of endogenous Topoisomerase I: whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with TOP1 / SCL-70 antibody Cat.-No. AM00154PU-N (0.5 µg/ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa. Lane 2: HepG2. Lane 3: HEK-293. Lane 4: SH-SY5Y. Lane 5: MDCK. Lane 6: PC12. Lane 7: CMT-93. Lane 8: Neuro 2A. Lane 9: 3T3.
