Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-10798 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-10798, RRID:AB_670083
- Product name
- Anti-MSH6
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references An intronic mutation in MLH1 associated with familial colon and breast cancer.
Lynch syndrome-associated mutations in MSH2 alter DNA repair and checkpoint response functions in vivo.
Bianchi F, Raponi M, Piva F, Viel A, Bearzi I, Galizia E, Bracci R, Belvederesi L, Loretelli C, Brugiati C, Corradini F, Baralle D, Cellerino R
Familial cancer 2011 Mar;10(1):27-35
Familial cancer 2011 Mar;10(1):27-35
Lynch syndrome-associated mutations in MSH2 alter DNA repair and checkpoint response functions in vivo.
Mastrocola AS, Heinen CD
Human mutation 2010 Oct;31(10):E1699-708
Human mutation 2010 Oct;31(10):E1699-708
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 153
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.