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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10813 - Provider product page
- Provider
- Abnova Corporation
- Product name
- MSH6 monoclonal antibody, clone 3E1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant MSH6.
- Isotype
- IgG
- Antibody clone number
- 3E1
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) against HEK293 (1), HCT116 (2), A-549 (3), A-431 (4), MCF-7 (5) and HepG2 (6) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) against human MSH6 (aa: 217-395) recombinant protein. (Expected MW is 160 kDa)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon cancer (A) and human rectum cancer (B) tissues using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using MSH6 monoclonal antibody, clone 3E1 (Cat # MAB10813) (green) and negative control (red).
- Validation comment
- Flow Cytometry
