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Learn18120-1-AP
antibody from Invitrogen Antibodies
Targeting: MSH6
GTBP
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [12]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 18120-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MSH6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSRQSTLYS FFPKSPALSD ANKASARASR EGGRAAAAPG ASPSPGGDAA WSEAGPGPRP LARSASPPKA KNLNGGLRRS VAPAAPTSCD FSPGDLVWAK MEGYPWWPCL VYNHPFDGTF IREKGKSVRV HVQFFDDSPT RGWVSKRLLK PYTGSKSKEA QKGGHFYSAK PEILRAMQRA DEALNKDKIK RLELAVCDEP SEPEEEEEME VGTTYVTDKS EEDNEIESEE EVQPKTQGSR RSSRQIKKRR VISDSESDIG GSDVEFKPDT KEEGSSDEIS SGVGDSESEG LNSPVKVARK RKRMVTGNGS LKRKSSRKET PSATKQATSI SSETKNTLRA FSAPQNSESQ AHVSGGGDDS SRPTVWYHET LEWLKEEKRR DEHRRRPDHP DFDASTLYVP E (1-400 aa encoded by BC004246)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.18 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 18120-1-AP (MSH6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of MSH6 antibody (18120-1-AP, 1:6000) with si-Control and si-MSH6 transfected HEK293 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 18120-1-AP (MSH6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 18120-1-AP (MSH6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using MSH6 antibody 18120-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 18120-1-AP (MSH6 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 18120-1-AP (MSH6 Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 18120-1-AP (MSH6 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 18120-1-AP (MSH6 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 18120-1-AP (MSH6 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 18120-1-AP (MSH6 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 18120-1-AP (MSH6 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 18120-1-AP (MSH6 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 18120-1-AP (MSH6 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 18120-1-AP (MSH6 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 18120-1-AP (MSH6 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 18120-1-AP ( MSH6 Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HEK-293 cells were intracellularly stained with 0.2 µg Anti-Human MSH6 (Product # 18120-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-MSH6 (IP:18120-1-AP, 4ug; Detection:18120-1-AP 1:600) with HEK-293 cells lysate 1120ug.
