Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX112986 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112986, RRID:AB_10617657
- Product name
- MyD88 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Mitochondrial‑DNA‑associated TLR9 signalling is a potential serological biomarker for non‑small cell lung cancer.
Lai YH, Liu HY, Huang CY, Chau YP, Wu S
Oncology reports 2019 Feb;41(2):999-1006
Oncology reports 2019 Feb;41(2):999-1006
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with MyD88 antibody (GTX112986) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ELISA detection of MyD88 using GTX112986 for capture at a concentration of 5 ?g/mL and GTX89605 for detection at a concentration of 5 ?g/mL.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MYD88 antibody detects MYD88 protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.Green: MYD88 protein stained by MYD88 antibody (GTX112986) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.