600-401-955

antibody from Rockland Immunochemicals, Inc.

Targeting: MYD88

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-955

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-955, RRID:AB_2250922

Product name

Anti-MyD88 (RABBIT) Antibody - 600-401-955

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Biochemical Isolation of the Myddosome from Murine Macrophages.

Tan Y, Kagan JC
Methods in molecular biology (Clifton, N.J.) 2018;1714:79-95

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-MyD88 antibody shows detection of MyD88 in Jurkat whole cell lysate.  The membrane was probed with the primary antibody diluted to 1:500. Background Information: MyD88 (Myeloid differentiation primary response protein).  The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway (1). MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and the dominant negative form of MyD88 attenuates IL-1R-mediated NF-kB activation.  MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor (2,3), both members of the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to the bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors.  MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. The MyD88 gene is expressed in many tissues.

Validation comment

Western Blot