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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00003843-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003843-M01, RRID:AB_547580
- Product name
- Mouse Monoclonal Karyopherin (importin) beta 3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RANBP5 - RAN binding protein 5
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Fas-Associated Factor 1 Negatively Regulates the Antiviral Immune Response by Inhibiting Translocation of Interferon Regulatory Factor 3 to the Nucleus.
Localization of retinitis pigmentosa 2 to cilia is regulated by Importin beta2.
Song S, Lee JJ, Kim HJ, Lee JY, Chang J, Lee KJ
Molecular and cellular biology 2016 Jan 25;36(7):1136-51
Molecular and cellular biology 2016 Jan 25;36(7):1136-51
Localization of retinitis pigmentosa 2 to cilia is regulated by Importin beta2.
Hurd TW, Fan S, Margolis BL
Journal of cell science 2011 Mar 1;124(Pt 5):718-26
Journal of cell science 2011 Mar 1;124(Pt 5):718-26
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01] - Analysis of IPO5 expression in transfected 293T cell line by RANBP5 monoclonal antibody (M01), clone 1C4.Lane 1: IPO5 transfected lysate (Predicted MW: 125.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01] - RANBP5 monoclonal antibody (M01), clone 1C4. Analysis of IPO5 expression in PC-12.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01] - Detection limit for recombinant GST tagged RANBP5 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01] - Analysis of monoclonal antibody to RANBP5 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
