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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00084254-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00084254-M01, RRID:AB_606017
- Product name
- CAMKK1 monoclonal antibody (M01), clone 1F6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant CAMKK1.
- Antigen sequence
MEGGPAVCCQDPRAELVERVAAIDVTHLEEADGGP
EPTRNGVDPPPRARAASVIPGSTSRLLPARPSLSA
RKLSLQERPAGSYLEAQAGPYATGPASHISPRAWR
RPTIESHHVAISDAEDCVQLNQYKLQSEIGKGAYG
VVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPP
PRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVN
VVKLIEVLDDPAEDNLYLALQNQAQNIQLDSTNIA
KPHSLLPSEQQDSGSTWAARSVFDLLRKGPVMEVP
CDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIK
PSNLLLGDDGHVKIADFGVSNQFEGNDAQLSSTAG
TPAFMAPEAISDSGQSFSGKALDVWATGVTLYCFV
YGKCPFIDDFILALHRKIKNEPVVFPEGPEISEEL
KDLILKMLDKNPETRIGVPDIKLHPWVTKNGEEPL
PSEEEHCSVVEVTEEEVKNSVRLIPSWTTVILVKS
MLRKRSFGNPFEPQARREERSMSAPGNLLV- Isotype
- IgG
- Antibody clone number
- 1F6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CAMKK1 monoclonal antibody (M01), clone 1F6 Western Blot analysis of CAMKK1 expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of CAMKK1 expression in transfected 293T cell line by CAMKK1 monoclonal antibody (M01), clone 1F6.Lane 1: CAMKK1 transfected lysate(57.31 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CAMKK1 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to CAMKK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
