Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00084254-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00084254-M01, RRID:AB_537379
- Product name
- Mouse Monoclonal CaMKK alpha Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CAMKK1 - calcium/calmodulin-dependent protein kinase kinase 1, alpha
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01] - CAMKK1 over-expressed 293 cell line. Lane 2 cotransfected with CAMKK1 Validated Chimera RNAi or non-transfected control. Blot probed with H00084254-M01. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01] - Analysis of CAMKK1 expression in transfected 293T cell line by CAMKK1 monoclonal antibody (M01), clone 1F6.Lane 1: CAMKK1 transfected lysate(57.31 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01] - CAMKK1 monoclonal antibody (M01), clone 1F6 Analysis of CAMKK1 expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CaMKK alpha Antibody (1F6) [H00084254-M01] - Detection limit for recombinant GST tagged CAMKK1 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CaMKK alpha Antibody (1F6) [H00084254-M01] - Analysis of monoclonal antibody to CAMKK1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1 ug/ml.