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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-42683 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-42683, RRID:AB_2068528
- Product name
- Rabbit Polyclonal CaMKK alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references LATS suppresses mTORC1 activity to directly coordinate Hippo and mTORC1 pathways in growth control.
Anti-apoptotic effects of PCP4/PEP19 in human breast cancer cell lines: a novel oncotarget.
Gan W, Dai X, Dai X, Xie J, Yin S, Zhu J, Wang C, Liu Y, Guo J, Wang M, Liu J, Hu J, Quinton RJ, Ganem NJ, Liu P, Asara JM, Pandolfi PP, Yang Y, He Z, Gao G, Wei W
Nature cell biology 2020 Feb;22(2):246-256
Nature cell biology 2020 Feb;22(2):246-256
Anti-apoptotic effects of PCP4/PEP19 in human breast cancer cell lines: a novel oncotarget.
Hamada T, Souda M, Yoshimura T, Sasaguri S, Hatanaka K, Tasaki T, Yoshioka T, Ohi Y, Yamada S, Tsutsui M, Umekita Y, Tanimoto A
Oncotarget 2014 Aug 15;5(15):6076-86
Oncotarget 2014 Aug 15;5(15):6076-86
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CaMKK alpha Antibody [NBP1-42683] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CaMKK alpha antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CaMKK alpha was also immunoprecipitated by rabbit anti-CaMKK alpha antibody NBP1-42682, which recognizes an upstream epitope.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CaMKK alpha Antibody [NBP1-42683] - Section of human lung carcinoma (left) and human prostate carcinoma (right). Antibody: Affinity purified rabbit anti- CaMKK alpha used at a dilution of 1:200 (1ug/ml). Detection: DAB
