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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- NB100-2834 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2834, RRID:AB_2193810
- Product name
- Goat Polyclonal Smad9 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is not expected to cross-react with SMAD1 and SMAD5.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Mechanisms of TGF-beta signaling from cell membrane to the nucleus.
Mechanisms of TGF-beta signaling from cell membrane to the nucleus.
Shi Y, Massagué J
Cell 2003 Jun 13;113(6):685-700
Cell 2003 Jun 13;113(6):685-700
Mechanisms of TGF-beta signaling from cell membrane to the nucleus.
Shi Y, Massagué J
Cell 2003 Jun 13;113(6):685-700
Cell 2003 Jun 13;113(6):685-700
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Smad9 Antibody [NB100-2834] - Analysis of Smad9 using NB100-2834 at 0.1 ug/ml in Human Skin lysate (35ug protein in RIPA buffer).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Smad9 Antibody [NB100-2834] - Antibody (1.5ug/ml) used as the reporter with a rabbit antibody (2.5ug/ml) used as capture. Recommended reporter concentration: 1-2ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Smad9 Antibody [NB100-2834] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
