GTX22774

antibody from GeneTex

Targeting: NR3C2 MLR, MR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

GTX22774

Provider

GeneTex

Proper citation

GeneTex Cat#GTX22774, RRID:AB_384844

Product name

Mineralocorticoid Receptor antibody [H10E4C9F]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Chicken/Avian, Rabbit, Sheep

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of Mineralocorticoid Receptor.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot of Mineralocorticoid Receptor using GTX22774

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of Mineralocorticoid Receptor in HEK293 cells. Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Mineralocorticoid Receptor antibody [H10E4C9F] at a dilution of 1:20-1:200 over night at 4 ¢XC, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemistry was performed on cancer biopsies of deparaffinized human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with or without Mineralocorticoid Receptor antibody [H10E4C9F] overnight at 4¢XC in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.