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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [19]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX116040 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116040, RRID:AB_2037097
- Product name
- GPX1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Changes in Metabolism, Mitochondrial Function, and Oxidative Stress Between Female Rats Under Nonreproductive and 3 Reproductive Conditions.
1,25-Dihydroxyvitamin D3 modulates the effects of sublethal BPA on mitochondrial function via activating PI3K-Akt pathway and 17β-estradiol secretion in rat granulosa cells.
Upregulation of Znf179 acetylation by SAHA protects cells against oxidative stress.
Impact of carbonylation on glutathione peroxidase-1 activity in human hyperglycemic endothelial cells.
Dietary Selenomethionine Administration and Its Effects on the American Alligator (Alligator mississippiensis): Oxidative Status and Corticosterone Levels.
A mitohormetic response to pro-oxidant exposure in the house mouse.
Modulation of glutathione peroxidase activity by age-dependent carbonylation in glomeruli of diabetic mice.
Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.
High activity before breeding improves reproductive performance by enhancing mitochondrial function and biogenesis.
3, 4-dihydroxybenzalacetone attenuates lipopolysaccharide-induced inflammation in acute lung injury via down-regulation of MMP-2 and MMP-9 activities through suppressing ROS-mediated MAPK and PI3K/AKT signaling pathways.
Unconventional role of voltage-gated proton channels (VSOP/Hv1) in regulation of microglial ROS production.
The 1-Week and 8-Month Effects of a Ketogenic Diet or Ketone Salt Supplementation on Multi-Organ Markers of Oxidative Stress and Mitochondrial Function in Rats.
ERRα Maintains Mitochondrial Oxidative Metabolism and Constitutes an Actionable Target in PGC1α-Elevated Melanomas.
Analyses of Selenotranscriptomes and Selenium Concentrations in Response to Dietary Selenium Deficiency and Age Reveal Common and Distinct Patterns by Tissue and Sex in Telomere-Dysfunctional Mice.
Lactation has persistent effects on a mother's metabolism and mitochondrial function.
Dietary selenium protects adiponectin knockout mice against chronic inflammation induced colon cancer.
Comparative changes in antioxidant enzymes and oxidative stress in cardiac, fast twitch and slow twitch skeletal muscles following endurance exercise training.
Reproduction Does Not Adversely Affect Liver Mitochondrial Respiratory Function but Results in Lipid Peroxidation and Increased Antioxidants in House Mice.
Shikonin inhibits oxidized LDL-induced monocyte adhesion by suppressing NFκB activation via up-regulation of PI3K/Akt/Nrf2-dependent antioxidation in EA.hy926 endothelial cells.
Hyatt HW, Zhang Y, Hood WR, Kavazis AN
Reproductive sciences (Thousand Oaks, Calif.) 2019 Jan;26(1):114-127
Reproductive sciences (Thousand Oaks, Calif.) 2019 Jan;26(1):114-127
1,25-Dihydroxyvitamin D3 modulates the effects of sublethal BPA on mitochondrial function via activating PI3K-Akt pathway and 17β-estradiol secretion in rat granulosa cells.
Lee CT, Wang JY, Chou KY, Hsu MI
The Journal of steroid biochemistry and molecular biology 2019 Jan;185:200-211
The Journal of steroid biochemistry and molecular biology 2019 Jan;185:200-211
Upregulation of Znf179 acetylation by SAHA protects cells against oxidative stress.
Wu CC, Lee PT, Kao TJ, Chou SY, Su RY, Lee YC, Yeh SH, Liou JP, Hsu TI, Su TP, Chuang CK, Chang WC, Chuang JY
Redox biology 2018 Oct;19:74-80
Redox biology 2018 Oct;19:74-80
Impact of carbonylation on glutathione peroxidase-1 activity in human hyperglycemic endothelial cells.
Sultan CS, Saackel A, Stank A, Fleming T, Fedorova M, Hoffmann R, Wade RC, Hecker M, Wagner AH
Redox biology 2018 Jun;16:113-122
Redox biology 2018 Jun;16:113-122
Dietary Selenomethionine Administration and Its Effects on the American Alligator (Alligator mississippiensis): Oxidative Status and Corticosterone Levels.
Finger JW Jr, Hamilton MT, Kelley MD, Zhang Y, Kavazis AN, Glenn TC, Tuberville TD
Archives of environmental contamination and toxicology 2018 Jul;75(1):37-44
Archives of environmental contamination and toxicology 2018 Jul;75(1):37-44
A mitohormetic response to pro-oxidant exposure in the house mouse.
Zhang Y, Humes F, Almond G, Kavazis AN, Hood WR
American journal of physiology. Regulatory, integrative and comparative physiology 2018 Jan 1;314(1):R122-R134
American journal of physiology. Regulatory, integrative and comparative physiology 2018 Jan 1;314(1):R122-R134
Modulation of glutathione peroxidase activity by age-dependent carbonylation in glomeruli of diabetic mice.
Wiedenmann T, Dietrich N, Fleming T, Altamura S, Deelman LE, Henning RH, Muckenthaler MU, Nawroth PP, Hammes HP, Wagner AH, Hecker M
Journal of diabetes and its complications 2018 Feb;32(2):130-138
Journal of diabetes and its complications 2018 Feb;32(2):130-138
Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.
Wu YL, Chang JC, Lin WY, Li CC, Hsieh M, Chen HW, Wang TS, Wu WT, Liu CS, Liu KL
Free radical biology & medicine 2018 Feb 1;115:309-317
Free radical biology & medicine 2018 Feb 1;115:309-317
High activity before breeding improves reproductive performance by enhancing mitochondrial function and biogenesis.
Zhang Y, Brasher AL, Park NR, Taylor HA, Kavazis AN, Hood WR
The Journal of experimental biology 2018 Apr 6;221(Pt 7)
The Journal of experimental biology 2018 Apr 6;221(Pt 7)
3, 4-dihydroxybenzalacetone attenuates lipopolysaccharide-induced inflammation in acute lung injury via down-regulation of MMP-2 and MMP-9 activities through suppressing ROS-mediated MAPK and PI3K/AKT signaling pathways.
Chao W, Deng JS, Huang SS, Li PY, Liang YC, Huang GJ
International immunopharmacology 2017 Sep;50:77-86
International immunopharmacology 2017 Sep;50:77-86
Unconventional role of voltage-gated proton channels (VSOP/Hv1) in regulation of microglial ROS production.
Kawai T, Okochi Y, Ozaki T, Imura Y, Koizumi S, Yamazaki M, Abe M, Sakimura K, Yamashita T, Okamura Y
Journal of neurochemistry 2017 Sep;142(5):686-699
Journal of neurochemistry 2017 Sep;142(5):686-699
The 1-Week and 8-Month Effects of a Ketogenic Diet or Ketone Salt Supplementation on Multi-Organ Markers of Oxidative Stress and Mitochondrial Function in Rats.
Kephart WC, Mumford PW, Mao X, Romero MA, Hyatt HW, Zhang Y, Mobley CB, Quindry JC, Young KC, Beck DT, Martin JS, McCullough DJ, D'Agostino DP, Lowery RP, Wilson JM, Kavazis AN, Roberts MD
Nutrients 2017 Sep 15;9(9)
Nutrients 2017 Sep 15;9(9)
ERRα Maintains Mitochondrial Oxidative Metabolism and Constitutes an Actionable Target in PGC1α-Elevated Melanomas.
Luo C, Balsa E, Thomas A, Hatting M, Jedrychowski M, Gygi SP, Widlund HR, Puigserver P
Molecular cancer research : MCR 2017 Oct;15(10):1366-1375
Molecular cancer research : MCR 2017 Oct;15(10):1366-1375
Analyses of Selenotranscriptomes and Selenium Concentrations in Response to Dietary Selenium Deficiency and Age Reveal Common and Distinct Patterns by Tissue and Sex in Telomere-Dysfunctional Mice.
Cao L, Zhang L, Zeng H, Wu RT, Wu TL, Cheng WH
The Journal of nutrition 2017 Oct;147(10):1858-1866
The Journal of nutrition 2017 Oct;147(10):1858-1866
Lactation has persistent effects on a mother's metabolism and mitochondrial function.
Hyatt HW, Zhang Y, Hood WR, Kavazis AN
Scientific reports 2017 Dec 7;7(1):17118
Scientific reports 2017 Dec 7;7(1):17118
Dietary selenium protects adiponectin knockout mice against chronic inflammation induced colon cancer.
Saxena A, Fayad R, Kaur K, Truman S, Greer J, Carson JA, Chanda A
Cancer biology & therapy 2017 Apr 3;18(4):257-267
Cancer biology & therapy 2017 Apr 3;18(4):257-267
Comparative changes in antioxidant enzymes and oxidative stress in cardiac, fast twitch and slow twitch skeletal muscles following endurance exercise training.
Hyatt HW, Smuder AJ, Sollanek KJ, Morton AB, Roberts MD, Kavazis AN
International journal of physiology, pathophysiology and pharmacology 2016;8(4):160-168
International journal of physiology, pathophysiology and pharmacology 2016;8(4):160-168
Reproduction Does Not Adversely Affect Liver Mitochondrial Respiratory Function but Results in Lipid Peroxidation and Increased Antioxidants in House Mice.
Mowry AV, Kavazis AN, Sirman AE, Potts WK, Hood WR
PloS one 2016;11(8):e0160883
PloS one 2016;11(8):e0160883
Shikonin inhibits oxidized LDL-induced monocyte adhesion by suppressing NFκB activation via up-regulation of PI3K/Akt/Nrf2-dependent antioxidation in EA.hy926 endothelial cells.
Huang CS, Lin AH, Yang TC, Liu KL, Chen HW, Lii CK
Biochemical pharmacology 2015 Feb 1;93(3):352-61
Biochemical pharmacology 2015 Feb 1;93(3):352-61
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GPX1 antibody detects GPX1 protein by western blot analysis.A. 30 ?g GL261 whole cell lysate/extract lysate/extractB. 30 ?g C8D30 whole cell lysate/extractC. 30 ?g NIH-3T3 whole cell lysate/extractD. 30 ?g Raw264.7 whole cell lysate/extractE. 30 ?g C2C12 whole cell lysate/extract12% SDS-PAGEGPX1 antibody (GTX116040) dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GPX1 antibody detects GPX1 protein by western blot analysis.A.50 ?g rat liver lysate/extract 12% SDS-PAGEGPX1 antibody (GTX116040) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Molt-4 (GTX27912) B: Raji 12% SDS PAGE GTX116040 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Glutathione peroxidase 1(GTX116040) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using GPX1(GTX116040) antibody at 1:100 dilution.
