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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP51937PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP51937PU-N, RRID:AB_11148351
- Product name
- anti Glutathione peroxidase 1 / GPX1 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 169-199aa) of human Glutathione peroxidase 1 / GPX1.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Glutathione peroxidase 1 / GPX1 (arrow) in mouse liver tissue lysates (35ug/lane) using Glutathione peroxidase 1 / GPX1 antibody Cat.-No. AP51937PU-N (C-term).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of GPX1 in HepG2 cells using Glutathione peroxidase 1 / GPX1 antibody Cat.-No. AP51937PU-N (C-term), followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human hepatocarcinoma tissue (Formalin-fixed, Paraffin-embedded) using Glutathione peroxidase 1 / GPX1 antibody Cat.-No. AP51937PU-N (C-term), followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram) using Glutathione peroxidase 1 / GPX1 antibody Cat.-No. AP51937PU-N (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
