- PA5-57575 - Invitrogen Antibodies TOMM40 antibody

Ha J, Park SB
Communications biology 2021 Sep 23;4(1):1123

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of TOMM40 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251 MG; Lane 4: Human plasma; Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a TOMM40 Polyclonal Antibody (Product # PA5-57575).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of TOMM40 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251 MG; Lane 4: Human plasma; Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a TOMM40 Polyclonal Antibody (Product # PA5-57575).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using TOMM40 Polyclonal Antibody (Product # PA5-57575) and a 39 kDa band corresponding to TOMM40 was observed across all the cell lines and tissues tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), SH-SY5Y (Lane 3), Neuro-2a (Lane 4), NK-92 (Lane 5), HEK-293 (Lane 6), SK-OV-3 (Lane 7), Mouse brain (Lane 8), Mouse liver (Lane 9) and Mouse heart (Lane 10) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent staining of TOMM40 in human cell line HEK 293 shows positivity in mitochondria. Samples were probed using a TOMM40 Polyclonal Antibody (Product # PA5-57575).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of TOMM40 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TOMM40 Polyclonal Antibody (Product # PA5-57575) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of TOMM40 in human rectal tissue shows strong granular cytoplasmic positivity in glandular cells. Samples were probed using a TOMM40 Polyclonal Antibody (Product # PA5-57575).

PA5-57575