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LearnR31034
antibody from NSJ Bioreagents
Targeting: SMARCB1
BAF47, hSNFS, Ini1, PPP1R144, RDT, Sfh1p, SNF5, SNF5L1, Snr1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- R31034 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SMARCB1 Antibody (BAF47)
- Antibody type
- Polyclonal
- Antigen
- Amino acids 362-377 (EKKIRDQDRNTRRMRR) were used as the immunogen for this SMARCB1 antibody (100% homologous in human, mouse and rat).
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the SMARCB1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing with SMARCB1 antibody; Lane 1: rat kidney; 2: human placenta; 3: (r) spleen; 4: (r) PC-12; 5: (h) HeLa; 6: (h) Jurkat; 7: (h) 293T cell lysate. Expected size ~47KD, observed ~70KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing with SMARCB1 antibody and recombinant protein, 41KD with tag; Lane 1: Recombinant protein 10ng; 2: 5ng; 3: 2.5ng
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: SNF5 antibody testing of human meningeoma tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: SMARCB1 antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
