sc-13088

antibody from Santa Cruz Biotechnology

Targeting: IHH BDA1, HHG2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-13088

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-13088, RRID:AB_2122007

Product name

Anti-IHH

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

References

Submitted references

Enhanced expression of hedgehog signaling molecules in squamous cell carcinoma of uterine cervix and its precursor lesions

Yan Hua Xuan, Hun Soon Jung, Yoon-La Choi, Young Kee Shin, Hee Jin Kim, Kyung Hee Kim, Wun Jae Kim, You Jeong Lee, Seok-Hyung Kim
Modern Pathology 2006 Jun;19(8):1139-1147

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 213, 118, 83, 50, 33, 26, 18

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 45

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human placenta shows strong cytoplasmic and nuclear positivity in trophoblastic cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.