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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB8048 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Indian Hedgehog/Ihh Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Indian Hedgehog/Ihh in ELISAs. In direct ELISAs, no cross-reactivity with full length recombinant mouse (rm) Dhh or rmShh is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P97812- Isotype
- IgG
- Antibody clone number
- 852526
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Alkaline Phosphatase Production Induced by Indian Hedgehog/Ihh and Neutral-ization by Mouse Indian Hedgehog/Ihh Antibody. Recombinant Human/Mouse Indian Hedgehog/Ihh (C28II), N-Terminus induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Indian Hedgehog/Ihh (10 ug/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Indian Hedgehog/Ihh Monoclonal Antibody (Catalog # MAB8048). The ND50 is typically 10-45 ug/mL.
