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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF1705 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Indian Hedgehog/Ihh N-Terminus Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Indian Hedgehog (Ihh) N-terminal peptide in Western blots. In Western blots, approximately 80% cross-reactivity with recombinant human Shh N-terminal peptide and recombinant mouse (rm) Shh N-terminal peptide is observed, and less than 1% cross-reactivity with rmIhh C-terminal peptide (amino acids 241-449) is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P97812- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Regulation of Calvarial Osteogenesis by Concomitant De-repression of GLI3 and Activation of IHH Targets.
Inhibition of p38 MAPK signaling in chondrocyte cultures results in enhanced osteogenic differentiation of perichondral cells.
Veistinen LK, Mustonen T, Hasan MR, Takatalo M, Kobayashi Y, Kesper DA, Vortkamp A, Rice DP
Frontiers in physiology 2017;8:1036
Frontiers in physiology 2017;8:1036
Inhibition of p38 MAPK signaling in chondrocyte cultures results in enhanced osteogenic differentiation of perichondral cells.
Stanton LA, Beier F
Experimental cell research 2007 Jan 1;313(1):146-55
Experimental cell research 2007 Jan 1;313(1):146-55
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Indian Hedgehog in Mouse Embryo. Indian Hedgehog was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse Indian Hedgehog N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1705) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
