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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF578 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IGFBP-5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IGFBP-5 in ELISAs and Western blots. In sandwich ELISAs, less than 3% cross-reactivity with recombinant human IGFBP-5 is observed, and less than 0.3% cross-reactivity with recombinant mouse (rm) IGF-I, rmIGF-II, rmIGFBP-1, rmIGFBP-2, rmIGFBP-3, and rmIGFBP-6 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q3UQV0- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Pappa2 deletion in mice affects male but not female fertility.
Christians JK, King AY, Rogowska MD, Hessels SM
Reproductive biology and endocrinology : RB&E 2015 Sep 29;13:109
Reproductive biology and endocrinology : RB&E 2015 Sep 29;13:109
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IGFBP-5 in Mouse Ovary. IGFBP-5 was detected in perfusion fixed frozen sections of mouse ovary (follicle with oocyte) using 5 µg/mL Goat Anti-Mouse IGFBP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF578) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
