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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-139 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-139, RRID:AB_632555
- Product name
- Anti-NTRK3
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Heterozygous NTF4 mutations impairing neurotrophin-4 signaling in patients with primary open-angle glaucoma.
TrkB signaling is required for both the induction and maintenance of tissue and nerve injury-induced persistent pain.
Truncated TrkB-T1 mediates neurotrophin-evoked calcium signalling in glia cells
Pasutto F, Matsumoto T, Mardin CY, Sticht H, Brandstätter JH, Michels-Rautenstrauss K, Weisschuh N, Gramer E, Ramdas WD, van Koolwijk LM, Klaver CC, Vingerling JR, Weber BH, Kruse FE, Rautenstrauss B, Barde YA, Reis A
American journal of human genetics 2009 Oct;85(4):447-56
American journal of human genetics 2009 Oct;85(4):447-56
TrkB signaling is required for both the induction and maintenance of tissue and nerve injury-induced persistent pain.
Wang X, Ratnam J, Zou B, England PM, Basbaum AI
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Apr 29;29(17):5508-15
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Apr 29;29(17):5508-15
Truncated TrkB-T1 mediates neurotrophin-evoked calcium signalling in glia cells
Christine R. Rose, Robert Blum, Bruno Pichler, Alexandra Lepier, Karl W. Kafitz, Arthur Konnerth
Nature 2003 Nov;426(6962):74-78
Nature 2003 Nov;426(6962):74-78
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 93
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
