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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [2]
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- Product number
- AF373 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TrkC Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TrkC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human TrkB is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q96CY4- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells.
TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells.
BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
Maury Y, Côme J, Piskorowski RA, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S
Nature biotechnology 2015 Jan;33(1):89-96
Nature biotechnology 2015 Jan;33(1):89-96
TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells.
Huang YT, Lai PC, Wu CC, Cheng CC, Chiu TH
International journal of oncology 2010 Oct;37(4):943-9
International journal of oncology 2010 Oct;37(4):943-9
TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells.
Huang YT, Lai PC, Wu CC, Cheng CC, Chiu TH
International journal of oncology 2010 Oct;37(4):943-9
International journal of oncology 2010 Oct;37(4):943-9
BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH
International journal of oncology 2010 Jun;36(6):1469-76
International journal of oncology 2010 Jun;36(6):1469-76
BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH
International journal of oncology 2010 Jun;36(6):1469-76
International journal of oncology 2010 Jun;36(6):1469-76
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of HAF017 TrkC by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human brain (hippocampus) tissue, human brain (hypothalamus) tissue, and human brain stem tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TrkC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF373) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TrkC at approximately 140, 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human TrkC by Simple WesternTM. Simple Western lane view shows lysates of human brain (hypothalamus) tissue, loaded at 0.2 mg/mL. A specific band was detected for TrkC at approximately 164 kDa (as indicated) using 10 µg/mL of Goat Anti-Human TrkC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF373) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
