368404

antibody from BioLegend

Targeting: IKZF1 hIk-1, Hs.54452, IKAROS, LyF-1, PPP1R92, ZNFN1A1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

368404

Provider

BioLegend

Proper citation

BioLegend Cat#368404, RRID:AB_2566183

Product name

Alexa Fluor® 647 anti-human Ikaros

Antibody type

Monoclonal

Antigen

Human Ikaros peptide (431-477 aa) conjugated to KLH Freund's adjuvant.

Description

Ikaros, also known as IKZF1, belongs to a family of zinc-finger DNA-binding proteins that regulate lymphocyte differentiation. It has the ability to form homo- and heterodimers with other members of the Ikaros family, which include Aiolos and Helios. Ikaros can be found on a majority of fetal and adult hematopoietic cell types such as T- and B-cells, natural killer cells (NKs), monocytes/macrophages, and dendritic cells. Ikaros is a tumor suppressor and its absence or reduced expression has been reported in several leukemias.

Reactivity

Human

Host

Mouse

Conjugate

Red dye

Antibody clone number

16B5C71

Vial size

100 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood mononuclear cells were stained with CD19 PE, treated with the True-Nuclear™ Transcription Factor Buffer Set, and then stained with Ikaros (clone 16B5C71) Alexa Fluor® 647 (top) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (bottom).

Conjugate

Red dye