Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 200-401-100 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-401-100, RRID:AB_2298500
- Product name
- Anti-NF-Y (A subunit) (RABBIT) Antibody - 200-401-100
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Infinity: An In-Silico Tool for Genome-Wide Prediction of Specific DNA Matrices in miRNA Genomic Loci.
NF-Y dependent epigenetic modifications discriminate between proliferating and postmitotic tissue.
Coordinated changes of histone modifications and HDAC mobilization regulate the induction of MHC class II genes by Trichostatin A.
Inhibition of major histocompatibility complex class II gene transcription by nitric oxide and antioxidants.
The MHC-specific enhanceosome and its role in MHC class I and beta(2)-microglobulin gene transactivation.
Orientation and positional mapping of the subunits of the multicomponent transcription factors RFX and X2BP to the major histocompatibility complex class II transcriptional enhancer.
Falcone E, Grandoni L, Garibaldi F, Manni I, Filligoi G, Piaggio G, Gurtner A
PloS one 2016;11(4):e0153658
PloS one 2016;11(4):e0153658
NF-Y dependent epigenetic modifications discriminate between proliferating and postmitotic tissue.
Gurtner A, Fuschi P, Magi F, Colussi C, Gaetano C, Dobbelstein M, Sacchi A, Piaggio G
PloS one 2008 Apr 23;3(4):e2047
PloS one 2008 Apr 23;3(4):e2047
Coordinated changes of histone modifications and HDAC mobilization regulate the induction of MHC class II genes by Trichostatin A.
Gialitakis M, Kretsovali A, Spilianakis C, Kravariti L, Mages J, Hoffmann R, Hatzopoulos AK, Papamatheakis J
Nucleic acids research 2006;34(3):765-72
Nucleic acids research 2006;34(3):765-72
Inhibition of major histocompatibility complex class II gene transcription by nitric oxide and antioxidants.
Grimm M, Spiecker M, De Caterina R, Shin WS, Liao JK
The Journal of biological chemistry 2002 Jul 19;277(29):26460-7
The Journal of biological chemistry 2002 Jul 19;277(29):26460-7
The MHC-specific enhanceosome and its role in MHC class I and beta(2)-microglobulin gene transactivation.
Gobin SJ, van Zutphen M, Westerheide SD, Boss JM, van den Elsen PJ
Journal of immunology (Baltimore, Md. : 1950) 2001 Nov 1;167(9):5175-84
Journal of immunology (Baltimore, Md. : 1950) 2001 Nov 1;167(9):5175-84
Orientation and positional mapping of the subunits of the multicomponent transcription factors RFX and X2BP to the major histocompatibility complex class II transcriptional enhancer.
Westerheide SD, Boss JM
Nucleic acids research 1999 Apr 1;27(7):1635-41
Nucleic acids research 1999 Apr 1;27(7):1635-41
No comments: Submit comment
Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit Anti-NF-Y(A subunit) Antibody. Lane 1: CHO-7 cells in the absence (I) of cholesterol. Lane 2: CHO-7 cells in the presence (S) of cholesterol. Load: Equivalent aliquots of chromatin from each sample containing ~50 µg of total protein per lane. Primary antibody: anti-NF-YA antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: doublet of ~ 42 kDa for NF-Y A.
- Validation comment
- Western Blot
