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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- A302-105A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NF-YA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse NF-YA by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-NF-YA antibody (Product # A302-105A; lot A302-105A-1) used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse NF-YA by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50µg) cells. Antibodies: Affinity purified rabbit anti-NF-YA antibody A302-105A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NF-YA was also immunoprecipitated by rabbit anti-NF-YA antibody A302-104A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 5 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse NF-YA by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse hybridoma tumor (right). Antibody: Affinity purified rabbit anti-NF-YA (Cat. No. A302-105A Lot1) used at a dilution of 1:200 (1 µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NF-YA by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NF-YA antibody (Product # A302-105A; lot A302-105A-1) used for IP at 3 µg/mg lysate. NF-YA was also immunoprecipitated by rabbit anti-NF-YA antibody (Product # A302-104A), which recognizes an upstream epitope. For blotting immunoprecipitated NF-YA (Product # A302-105A) was used at 1.0 µg/mL. Detection: Chemiluminescence with exposure times of 5 seconds.
