Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB600-481 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-481, RRID:AB_2152076
- Product name
- Rabbit Polyclonal NFYA Antibody
- Antibody type
- Polyclonal
- Description
- Delipidation and Defibrination. Recognizes the A subunit of human and mouse NFY. No reaction was observed against the B subunit of NFY.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.05 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NFYA Antibody [NB600-481] - Lane 1: CHO-7 cells were cultured in the absence (I) of cholesterol. Lane 2: CHO-7 cells were cultured in the presence (S) of cholesterol. Load: 35 ug per lane. Primary antibody: NF-YA antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: NF-YA recognized as a doublet of 42 kDa in size. Other band(s): none.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: NFYA Antibody [NB600-481] - Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: NF-Y antibody at 10ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: NF-Y is nuclear. Staining: NF-Y subunit A as precipitated red signal with hematoxylin purple nuclear counterstain.
