12037-1-AP

antibody from Invitrogen Antibodies

Targeting: DVL2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12037-1-AP

Provider

Invitrogen Antibodies

Product name

DVL2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAGSSTGGG GVGETKVIYH LDEEETPYLV KIPVPAERIT LGDFKSVLQR PAGAKYFFKS MDQDFGVVKE EISDDNARLP CFNGRVVSWL VSSDNPQPEM APPVHEPRAE LAPPAPPLPP LPPERTSGIG DSRPPSFHPN VSSSHENLEP ETETESVVSL RRERPRRRDS SEHGAGGHRT GGPSRLERHL AGYESSSTLM TSELESTSLG DSDEEDTMSR FSSSTEQSSA SRLLKRHRRR RKQRPPRLER TSSFSSVTDS TMSLNIITVT LNMEKYNFLG ISIVGQSNER GDGGIYIGSI MKGGAVAADG RIEPGDMLLQ VNDMNFENMS NDDAVRVLRD I (1-340 aa encoded by BC014844)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.41 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of DVL2 antibody (12037-1-AP, 1:1000) with siDVL2 transfected cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF-7 cells were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 12037-1-AP (DVL2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IGG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 12037-1-AP (DVL2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DVL2 (IP:12037-1-AP, 4ug; Detection:12037-1-AP 1:1000) with MCF-7 cells lysate 3200ug.