- PA5-27965 - Invitrogen Antibodies DVL2 antibody

Submitted references Islr regulates canonical Wnt signaling-mediated skeletal muscle regeneration by stabilizing Dishevelled-2 and preventing autophagy.

Zhang K, Zhang Y, Gu L, Lan M, Liu C, Wang M, Su Y, Ge M, Wang T, Yu Y, Liu C, Li L, Li Q, Zhao Y, Yu Z, Wang F, Li N, Meng Q
Nature communications 2018 Dec 3;9(1):5129

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of DVL2 was performed by separating 50 µg of whole cell extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a DVL2 Polyclonal Antibody (Product # PA5-27965) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot using DVL2 Polyclonal Antibody (Product # PA5-27965). Sample (30 µg whole cell lysate). A: MOLT4. B: Raji. 7.5% SDS PAGE. DVL2 Polyclonal Antibody (Product # PA5-27965) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded human lung SCC, using Dishevelled 2 (Product # PA5-27965) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 8 The ablation of Dact1 rescues the impaired skeletal muscle regeneration of Islr cKO mice by upregulating Dvl2. a Immunohistochemistry analysis of Islr and Dvl2 in injured TA muscles of WT mice at 5 d postinjury using serial sections. Scale bar = 10 mum. b Immunohistochemistry analysis of Islr and beta-catenin in injured TA muscles of WT mice at 5 d postinjury using serial sections. Scale bar = 10 mum. c Western blot analysis of Dvl2 protein levels in injured and CTL of control and Islr cKO mice at 3.5 d post injury. d Western blot analysis of Dvl2 and Axin1 protein levels in isolated satellite cells of control and Islr cKO mice cultured for 2 d in differentiation medium. e Intramuscular injection of CHIR or BSA at 2.5 d postinjury and H&E staining of injured TA muscles of control and Islr cKO mice at 5 d postinjury. N = 3 in each group. Scale bar = 100 mum . f CSA of myofibers in ( e ). g Western blot analysis of Dvl2 protein levels in injured TA muscles of control, Islr cKO, Dact1 cKO, and Islr / Dact1 cKO mice at 5 d postinjury. h Western blot analysis of active beta-catenin protein levels in injured TA muscles of control, Islr cKO, Dact1 cKO, and Islr / Dact1 cKO mice at 5 d postinjury. i H&E staining of injured and contralateral TA muscles in control, Islr cKO, Dact1 cKO, and Islr / Dact1 cKO mice at 5 d postinjury. Control (Ctrl): N = 5; Islr cKO: N = 3; Dact1 cKO: N = 3; Islr / Dact1 cKO: N =

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 9 Islr stabilizes Dvl2 via antagonizing LC3-mediated autophagy. a Immunofluorescence analysis of GFP-LC3 + puncta in C2C12 cells transfected with GFP-LC3 and Dvl2-Flag plasmids, GFP-LC3 and Islr-HA plasmids, or GFP-LC3, Dvl2-Flag, and Islr-HA plasmids. N = 3 cell cultures in each group. Approximately, 20 cells total in each group. The numbers of GFP-LC3 + puncta per cell are shown on the right. b Western blot analysis of p62 and LC3II protein levels in shCtrl and sh Islr C2C12 cells after 2 d in growth medium. c Immunofluorescence analysis of GFP-LC3 + /Dvl2-Flag + puncta in shCtrl and sh Islr C2C12 cells transfected with GFP-LC3 and Dvl2-Flag plasmids. N = 3 cell cultures in each group. Approximately, 20 cells total in each group. The numbers of GFP-LC3 + /Dvl2-Flag + puncta per cell are shown on the right. d Immunofluorescence analysis of GFP-LC3 + /Dvl2 + puncta in shCtrl and sh Islr C2C12 cells treated with rapamycin (Rap) for 6 h. N = 3 cell cultures in each group. Approximately, 15 cells total in each group. The numbers of GFP-LC3 + /Dvl2 + puncta per cell are shown on the right. e Immunofluorescence analysis of GFP-LC3 + /Dvl2 + puncta in satellite cells from control, Islr cKO, Dact1 cKO, and Islr / Dact1 cKO mice treated with rapamycin (Rap) for 6 h. N = 3 cell cultures in each group. Approximately, 15 cells total in each group. The numbers of GFP-LC3 + /Dvl2 + puncta per cell are shown on the right. Scale bars are all 2

PA5-27965

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