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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB4094 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat FKBP51 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects endogenous human, mouse and rat FKBP51 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q13451- Isotype
- IgG
- Antibody clone number
- 425314
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Neuropsin cleaves EphB2 in the amygdala to control anxiety.
Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R
Nature 2011 May 19;473(7347):372-5
Nature 2011 May 19;473(7347):372-5
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human and Mouse FKBP51 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP51 Monoclonal Antibody (Catalog # MAB4094) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP51 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
