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- Antigen structure
- References [2]
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- Western blot [3]
- Flow cytometry [1]
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- Product number
- NB100-804 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-804, RRID:AB_2064926
- Product name
- Goat Polyclonal BLNK Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize both reported isoforms (NP_037446.1
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references BLNK: a central linker protein in B cell activation.
BLNK: a central linker protein in B cell activation.
Fu C, Turck CW, Kurosaki T, Chan AC
Immunity 1998 Jul;9(1):93-103
Immunity 1998 Jul;9(1):93-103
BLNK: a central linker protein in B cell activation.
Fu C, Turck CW, Kurosaki T, Chan AC
Immunity 1998 Jul;9(1):93-103
Immunity 1998 Jul;9(1):93-103
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BLNK Antibody [NB100-804] - (4 ug/ml) of Daudi lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BLNK Antibody [NB100-804] - HEK293 overexpressing BLNK (RC202488) and probed with NB100-804 (mock transfection in first lane).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BLNK Antibody [NB100-804] - Staining of Daudi cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: BLNK Antibody [NB100-804] - Flow cytometric analysis of paraformaldehyde fixed Daudi cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
