Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PB9967 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-YAP1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for YAP/YAP1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, ICC/IF, FCM. Reactive species: Human;Mouse;Rat. YAP/YAP1 information: Molecular Weight: 54462 MW; Subcellular Localization: Cytoplasm . Nucleus . Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density (PubMed:18158288, PubMed:20048001). PTPN14 induces translocation from the nucleus to the cytoplasm (PubMed:22525271); Tissue Specificity: Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Alpha-lipoic Acid Prevents Bone Loss in Type 2 Diabetes and Postmenopausal Osteoporosis Coexisting Conditions by Modulating the YAP/Glut4 Pathway.
Synthesis, biological activity and mechanism of action of novel allosecurinine derivatives as potential antitumor agents.
Periodic Heat Stress Licenses EMSC Differentiation into Osteoblasts via YAP Signaling Pathway Activation.
Enhanced Bone Regeneration Using a ZIF-8-Loaded Fibrin Composite Scaffold.
Effect of Gestational Diabetes on Postpartum Depression-like Behavior in Rats and Its Mechanism.
YAP accelerates vascular senescence via blocking autophagic flux and activating mTOR.
Identification and differentiation therapy strategy of pterygium in vitro.
Xu L, Zhang C, Bao J, Han G, Wang C, Cai Y, Xu G, Sun H, Liu M
Cell biochemistry and biophysics 2024 Jun;82(2):669-685
Cell biochemistry and biophysics 2024 Jun;82(2):669-685
Synthesis, biological activity and mechanism of action of novel allosecurinine derivatives as potential antitumor agents.
Xu XL, Lan JX, Huang H, Dai W, Peng XP, Liu SL, Chen WM, Huang LJ, Liu J, Li XJ, Zeng JL, Huang XH, Zhao GN, Hou W
Bioorganic & medicinal chemistry 2023 Mar 15;82:117234
Bioorganic & medicinal chemistry 2023 Mar 15;82:117234
Periodic Heat Stress Licenses EMSC Differentiation into Osteoblasts via YAP Signaling Pathway Activation.
Shi W, Wang Z, Bian L, Wu Y, HuiYa M, Zhou Y, Zhang Z, Wang Q, Zhao P, Lu X
Stem cells international 2022;2022:3715471
Stem cells international 2022;2022:3715471
Enhanced Bone Regeneration Using a ZIF-8-Loaded Fibrin Composite Scaffold.
Shi W, Bian L, Wu Y, Wang Z, Dai Y, Zhou Y, Meng P, Wang Q, Zhang Z, Zhao X, Zhao P, Lu X
Macromolecular bioscience 2022 Mar;22(3):e2100416
Macromolecular bioscience 2022 Mar;22(3):e2100416
Effect of Gestational Diabetes on Postpartum Depression-like Behavior in Rats and Its Mechanism.
Zhao R, Zhou Y, Shi H, Ye W, Lyu Y, Wen Z, Li R, Xu Y
Nutrients 2022 Mar 14;14(6)
Nutrients 2022 Mar 14;14(6)
YAP accelerates vascular senescence via blocking autophagic flux and activating mTOR.
Pan X, Wu B, Fan X, Xu G, Ou C, Chen M
Journal of cellular and molecular medicine 2021 Jan;25(1):170-183
Journal of cellular and molecular medicine 2021 Jan;25(1):170-183
Identification and differentiation therapy strategy of pterygium in vitro.
Hu X, Tandra N, Zhang Z, Gong A, Chen J, Li Y, Chen Q, Xu W, Qian H
American journal of translational research 2018;10(8):2619-2627
American journal of translational research 2018;10(8):2619-2627
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of YAP1 expression in rat testis extract (lane 1), mouse ovary extract (lane 2), HELA whole cell lysates (lane 3) and MCF-7 whole cell lysates (lane 4). YAP1 at 54KD; 70KD was detected using rabbit anti-YAP1 Antigen Affinity purified polyclonal antibody (Catalog # PB9967) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
- Additional image