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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- STJP000324 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Human Interferon-beta 1b protein (Recombinant) (STJP000324)
- Antibody type
- Other
- Description
- Recombinant-Human Interferon-beta 1b-protein was developed from cho cells. For use in research applications.
- Conjugate
- Unconjugated
- Antigen sequence
MKYTSYILAF QLCIVLGSLG CYCQDPYVKE AE
NLKKYFNA GHSDVADNGT LFLGILKNWK EESD
RKIMQS QIVSFYFKLF KNFKDDQSIQ KSVETI
KEDM NVKFFNSNKK KRDDFEKLTN YSVTDLNV
QR KAIHELIQVM AELSPAAKTG KRKRSQMLFR
GRRASQ- Epitope
- NA
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- The lyophilized protein is stable for at least years from date of receipt at-20°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8°C for one month, or at-20°C for six months, with a carrier protein without detectable loss
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- E) Recombinant Type III Interferons in the absence of CM at the same concentrations as found in the CM (IL-28A/IFN Lambda 2: 1500 pg/mL; IL-28B/IFN Lambda 3: 10 pg/mL; IL-29/IFN Lambda 1: 500 pg/mL) were added to JFH-1 infected Huh7. 5. 1 cells.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Venous blood was collected prior to chemotherapy or at least one month following chemotherapy. For the offspring (HLA haploidentical donors) , the routine blood tests, and liver and kidney function tests were normal and negative for hepatitis A virus-IgM, hepatitis B surface antigen antibody, hepatitis B e--hepatitis B core-hepatitis C virus (HCV) -HCV-IgG, Syphilis-and human immunodeficiency virus-. As reported in our previous study (2 atmosphere. On the day of culture, 1, 000 U/ml human recombinant interferon-Gamma, 500 U/ml recombinant human interleukin (rhIL) -1 Alpha and 1, 000 U/ml rhIL-2 (Quangang Pharmaceutical Co. , , , ) were added. Four days later, 1, 000 U/ml rhIL-2 was added and the cells were transferred into a GT-T610 culture bag. Cell growth was observed every other day and cells were stained with 0. 4% trypan blue and the viable cells were counted. Following 18 days of culture, cells were infused once daily (>1×109 cells; viability rate, >95%) . A cycle of treatment…
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
