Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-77288 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77288, RRID:AB_11039065
- Product name
- Rabbit Polyclonal IFN-beta Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The early local and systemic Type I interferon responses to ultraviolet B light exposure are cGAS dependent.
Tumor microenvironment and RIG-I signaling molecules in Epstein Barr virus-positive and -negative classical Hodgkin lymphoma of the elderly.
Tumor microenvironment and RIG-I signaling molecules in Epstein Barr virus-positive and -negative classical Hodgkin lymphoma of the elderly.
Interferon-beta treatment and the natural history of relapsing-remitting multiple sclerosis.
TLR pathways and IFN-regulatory factors: to each its own.
Wherefore interferon?
Wherefore interferon?
Skopelja-Gardner S, An J, Tai J, Tanaka L, Sun X, Hermanson P, Baum R, Kawasumi M, Green R, Gale M Jr, Kalus A, Werth VP, Elkon KB
Scientific reports 2020 May 13;10(1):7908
Scientific reports 2020 May 13;10(1):7908
Tumor microenvironment and RIG-I signaling molecules in Epstein Barr virus-positive and -negative classical Hodgkin lymphoma of the elderly.
Satoh T, Wada R, Yajima N, Imaizumi T, Yagihashi S
Journal of clinical and experimental hematopathology : JCEH 2014;54(1):75-84
Journal of clinical and experimental hematopathology : JCEH 2014;54(1):75-84
Tumor microenvironment and RIG-I signaling molecules in Epstein Barr virus-positive and -negative classical Hodgkin lymphoma of the elderly.
Satoh T, Wada R, Yajima N, Imaizumi T, Yagihashi S
Journal of clinical and experimental hematopathology : JCEH 2014;54(1):75-84
Journal of clinical and experimental hematopathology : JCEH 2014;54(1):75-84
Interferon-beta treatment and the natural history of relapsing-remitting multiple sclerosis.
Koch M, Mostert J, De Keyser J, Tremlett H, Filippini G
Annals of neurology 2008 Jan;63(1):125-6; author reply 126-7
Annals of neurology 2008 Jan;63(1):125-6; author reply 126-7
TLR pathways and IFN-regulatory factors: to each its own.
Colonna M
European journal of immunology 2007 Feb;37(2):306-9
European journal of immunology 2007 Feb;37(2):306-9
Wherefore interferon?
Gresser I
Journal of leukocyte biology 1997 May;61(5):567-74
Journal of leukocyte biology 1997 May;61(5):567-74
Wherefore interferon?
Gresser I
Journal of leukocyte biology 1997 May;61(5):567-74
Journal of leukocyte biology 1997 May;61(5):567-74
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IFN-beta Antibody [NBP1-77288] - Analysis in A-20 cell lysate with antibody at 5 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IFN-beta Antibody [NBP1-77288] - Loading: 15 ug of lysates per lane. Antibodies: IFN-beta (4 ug/mL), IFN-beta , (5 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IFN-beta Antibody [NBP1-77288] - Loading: 15 ug of lysates per lane. Antibodies: IFN-beta (4 ug/mL), IFN-beta (5 ug/mL) and beta-actin(1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IFN-beta Antibody [NBP1-77288] - Validation in Multiple Tissues and Recombinant ProteinLoading: 15 ug of lysates per lane. Antibodies: IFN-beta (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IFN-beta Antibody [NBP1-77288] - Analysis of paraffin-embedded human liver tissue using anti-IFN-beta at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IFN-beta Antibody [NBP1-77288] - Analysis of paraffin-embedded human liver tissue using anti-IFN-beta antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.