500-P32B-100UG

antibody from Invitrogen Antibodies

Targeting: IFNB1 IFB, IFF, IFNB

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P32B-100UG

Provider

Invitrogen Antibodies

Product name

IFN beta Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN.

Concentration

0.1-1.0 mg/mL

References

Submitted references

PARP7 negatively regulates the type I interferon response in cancer cells and its inhibition triggers antitumor immunity.

Gozgit JM, Vasbinder MM, Abo RP, Kunii K, Kuplast-Barr KG, Gui B, Lu AZ, Molina JR, Minissale E, Swinger KK, Wigle TJ, Blackwell DJ, Majer CR, Ren Y, Niepel M, Varsamis ZA, Nayak SP, Bamberg E, Mo JR, Church WD, Mady ASA, Song J, Utley L, Rao PE, Mitchison TJ, Kuntz KW, Richon VM, Keilhack H
Cancer cell 2021 Sep 13;39(9):1214-1226.e10

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Respiratory infections regulated blood cells IFN-β-PD-L1 pathway in pediatric asthma.

Kölle J, Haag P, Vuorinen T, Alexander K, Rauh M, Zimmermann T, Papadopoulos NG, Finotto S
Immunity, inflammation and disease 2020 Sep;8(3):310-319

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STAT3/p53 pathway activation disrupts IFN-β-induced dormancy in tumor-repopulating cells.

Liu Y, Lv J, Liu J, Liang X, Jin X, Xie J, Zhang L, Chen D, Fiskesund R, Tang K, Ma J, Zhang H, Dong W, Mo S, Zhang T, Cheng F, Zhou Y, Jia Q, Zhu B, Kong Y, Guo J, Zhang H, Hu ZW, Cao X, Qin FX, Huang B
The Journal of clinical investigation 2018 Mar 1;128(3):1057-1073

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Activated Tissue-Resident Mesenchymal Stromal Cells Regulate Natural Killer Cell Immune and Tissue-Regenerative Function.

Petri RM, Hackel A, Hahnel K, Dumitru CA, Bruderek K, Flohe SB, Paschen A, Lang S, Brandau S
Stem cell reports 2017 Sep 12;9(3):985-998

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The oncolytic virus dl922-947 reduces IL-8/CXCL8 and MCP-1/CCL2 expression and impairs angiogenesis and macrophage infiltration in anaplastic thyroid carcinoma.

Passaro C, Borriello F, Vastolo V, Di Somma S, Scamardella E, Gigantino V, Franco R, Marone G, Portella G
Oncotarget 2016 Jan 12;7(2):1500-15

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Type I interferon induces CX3CL1 (fractalkine) and CCL5 (RANTES) production in human pulmonary vascular endothelial cells.

Nakano M, Fujii T, Hashimoto M, Yukawa N, Yoshifuji H, Ohmura K, Nakaizumi A, Mimori T
Clinical and experimental immunology 2012 Oct;170(1):94-100

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human IFN-beta by Western Blot analysis IFN beta Polyclonal Antibody (Product # 500-P32B-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IFN-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human IFN-beta by Western Blot analysis IFN beta Polyclonal Antibody (Product # 500-P32B-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IFN-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human IFN-beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IFN beta Polyclonal Antibody (Product # 500-P32B-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IFN beta Polyclonal Antibody, Biotin (Product # 500-P32BBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IFN-beta.