APR00512G

antibody from Leading Biology

Targeting: C3AR1 AZ3B, C3AR

Western blot (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

APR00512G

Provider

Leading Biology

Product name

C3a Anaphylatoxin Receptor (extracellular) Antibody

Antibody type

Polyclonal

Description

The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages1. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex1.Three different pathways are known to activate the complement. The C3 complement plays a key role in all three pathways leading to the activation of the complement. Produced in the liver, C3 is the most abundant complement protein in the serum. It is composed of two polypeptides, an α and β chain. Cleavage of C3 leads to the production of C3b and C3a1.C3a binds to C3a receptor, a G-protein coupled receptor which couples to Gi upon activation. Like all other members of the superfamily, C3a receptor has seven transmembrane domains with an extracellular N-terminus and intracellular C-terminal tail1. The receptor has two glycosylation sites, an unusually large extracellular loop with a sulfated tyrosine residue important for ligand recognition and binding2,3.C3a receptor is expressed in cells of myeloid origin like neutrophils, macrophages and dendritic cells to name a few2. It is also detected in astrocytes from inflamed brain4, endothelial cells5 and activated human T cells6. mRNA of C3a receptor is detected in lung, liver, kidney, brain heart, muscle and testis2,7.Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of human C3a receptor. Anti-C3a Anaphylatoxin Receptor (extracellular) antibody (#AG1373) can be used in western blot and indirect flow cytometry applications. It has been designed to recognize C3a receptor from human samples.

Reactivity

Human

Host

Rabbit

Storage

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.