HPA001403

antibody from Atlas Antibodies

Targeting: SIX6 OPTX2, Six9

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA001403

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001403, RRID:AB_1079993

Product name

Anti-SIX6

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

YQEAEKLRGRPLGPVDKYRVRKKFPLPRTIWDGEQ
KTHCFKERTRNLLREWYLQDPYPNPSKKRELAQAT
GLTPTQVGNWFKNRRQRDRAAAAKNRLQQQVLSQG
SGRALRAEGDGTPEVLGVATSPAASLSSKAATSAI
SITSSDSECDI


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and SIX6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416023).

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human eye tissue.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pituitary gland shows moderate nuclear positivity in anterior cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human retina, inner nuclear layer, shows moderate nuclear positivity.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pituitary gland shows moderate nuclear positivity.

Sample type

HUMAN