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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-204 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-204, RRID:AB_632480
- Product name
- Anti-TBP
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Role for the MOV10 RNA helicase in polycomb-mediated repression of the INK4a tumor suppressor.
TLS inhibits RNA polymerase III transcription.
SWI/SNF associates with nascent pre-mRNPs and regulates alternative pre-mRNA processing.
c-Myc induces changes in higher order rDNA structure on stimulation of quiescent cells
A unifying model for the selective regulation of inducible transcription by CpG islands and nucleosome remodeling.
Potential role of PUMA in delayed death of hippocampal CA1 neurons after transient global cerebral ischemia.
A reconfigured pattern of MLL occupancy within mitotic chromatin promotes rapid transcriptional reactivation following mitotic exit.
Elevated ATF4 expression, in the absence of other signals, is sufficient for transcriptional induction via CCAAT enhancer-binding protein-activating transcription factor response elements.
Despite increased ATF4 binding at the C/EBP-ATF composite site following activation of the unfolded protein response, system A transporter 2 (SNAT2) transcription activity is repressed in HepG2 cells.
Mutant Huntingtin reduces HSP70 expression through the sequestration of NF-Y transcription factor.
Variations in intracellular levels of TATA binding protein can affect specific genes by different mechanisms.
p53-Dependent p21 mRNA elongation is impaired when DNA replication is stalled.
Reduced oxidative stress promotes NF-kappaB-mediated neuroprotective gene expression after transient focal cerebral ischemia: lymphocytotrophic cytokines and antiapoptotic factors.
Vaccinia virus intermediate and late promoter elements are targeted by the TATA-binding protein.
Recruitment and the role of nuclear localization in polyglutamine-mediated aggregation.
El Messaoudi-Aubert S, Nicholls J, Maertens GN, Brookes S, Bernstein E, Peters G
Nature structural & molecular biology 2010 Jul;17(7):862-8
Nature structural & molecular biology 2010 Jul;17(7):862-8
TLS inhibits RNA polymerase III transcription.
Tan AY, Manley JL
Molecular and cellular biology 2010 Jan;30(1):186-96
Molecular and cellular biology 2010 Jan;30(1):186-96
SWI/SNF associates with nascent pre-mRNPs and regulates alternative pre-mRNA processing.
Tyagi A, Ryme J, Brodin D, Ostlund Farrants AK, Visa N
PLoS genetics 2009 May;5(5):e1000470
PLoS genetics 2009 May;5(5):e1000470
c-Myc induces changes in higher order rDNA structure on stimulation of quiescent cells
C-N Shiue, R G Berkson, A P H Wright
Oncogene 2009 Mar;28(16):1833-1842
Oncogene 2009 Mar;28(16):1833-1842
A unifying model for the selective regulation of inducible transcription by CpG islands and nucleosome remodeling.
Ramirez-Carrozzi VR, Braas D, Bhatt DM, Cheng CS, Hong C, Doty KR, Black JC, Hoffmann A, Carey M, Smale ST
Cell 2009 Jul 10;138(1):114-28
Cell 2009 Jul 10;138(1):114-28
Potential role of PUMA in delayed death of hippocampal CA1 neurons after transient global cerebral ischemia.
Niizuma K, Endo H, Nito C, Myer DJ, Chan PH
Stroke 2009 Feb;40(2):618-25
Stroke 2009 Feb;40(2):618-25
A reconfigured pattern of MLL occupancy within mitotic chromatin promotes rapid transcriptional reactivation following mitotic exit.
Blobel GA, Kadauke S, Wang E, Lau AW, Zuber J, Chou MM, Vakoc CR
Molecular cell 2009 Dec 25;36(6):970-83
Molecular cell 2009 Dec 25;36(6):970-83
Elevated ATF4 expression, in the absence of other signals, is sufficient for transcriptional induction via CCAAT enhancer-binding protein-activating transcription factor response elements.
Shan J, Ord D, Ord T, Kilberg MS
The Journal of biological chemistry 2009 Aug 7;284(32):21241-8
The Journal of biological chemistry 2009 Aug 7;284(32):21241-8
Despite increased ATF4 binding at the C/EBP-ATF composite site following activation of the unfolded protein response, system A transporter 2 (SNAT2) transcription activity is repressed in HepG2 cells.
Gjymishka A, Palii SS, Shan J, Kilberg MS
The Journal of biological chemistry 2008 Oct 10;283(41):27736-27747
The Journal of biological chemistry 2008 Oct 10;283(41):27736-27747
Mutant Huntingtin reduces HSP70 expression through the sequestration of NF-Y transcription factor.
Yamanaka T, Miyazaki H, Oyama F, Kurosawa M, Washizu C, Doi H, Nukina N
The EMBO journal 2008 Mar 19;27(6):827-39
The EMBO journal 2008 Mar 19;27(6):827-39
Variations in intracellular levels of TATA binding protein can affect specific genes by different mechanisms.
Bush SD, Richard P, Manley JL
Molecular and cellular biology 2008 Jan;28(1):83-92
Molecular and cellular biology 2008 Jan;28(1):83-92
p53-Dependent p21 mRNA elongation is impaired when DNA replication is stalled.
Mattia M, Gottifredi V, McKinney K, Prives C
Molecular and cellular biology 2007 Feb;27(4):1309-20
Molecular and cellular biology 2007 Feb;27(4):1309-20
Reduced oxidative stress promotes NF-kappaB-mediated neuroprotective gene expression after transient focal cerebral ischemia: lymphocytotrophic cytokines and antiapoptotic factors.
Song YS, Lee YS, Narasimhan P, Chan PH
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2007 Apr;27(4):764-75
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2007 Apr;27(4):764-75
Vaccinia virus intermediate and late promoter elements are targeted by the TATA-binding protein.
Knutson BA, Liu X, Oh J, Broyles SS
Journal of virology 2006 Jul;80(14):6784-93
Journal of virology 2006 Jul;80(14):6784-93
Recruitment and the role of nuclear localization in polyglutamine-mediated aggregation.
Perez MK, Paulson HL, Pendse SJ, Saionz SJ, Bonini NM, Pittman RN
The Journal of cell biology 1998 Dec 14;143(6):1457-70
The Journal of cell biology 1998 Dec 14;143(6):1457-70
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 38
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells and glial cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
