Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS5860 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- PLC ÉÇ2 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 382-427 of Human PLC ÉÇ2.
- Description
- Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC ÉÇ1, PLC ÉÇ2, PLC ÉÇ3, PLC ÉÇ4, PLC ÎÛ1, PLC ÎÛ2, PLC Ñ´1,PLC Ñ´2 and PLC ÷). PLC ÉÇs are the only PLC isforms that are regulated by G protein subunits and are activated by a heterotrimeric GTP-binding protein linked to various cell surface receptors. Two alternatively spliced forms (1,181 and 1,166 amino acids) of PLC ÉÇ2 are generated in hematopoietic cells that differ in the carboxyl-terminal sequence implicated in interaction of PLC ÉÇ enzymes with GÏ«q.The pleckstrin homology domain of PLC ÉÇ2 is required for its targeting to the membrane and for substrate hydrolysis and its linker region exerts an inhibitory efect on basal PLC ÉÇ2 activity. PLC ÉÇ2 plays a major role in platelet activation and is mainly expressed in the periphery of the islet and acinar cells in rat pancreas.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of PLC ¦Â2 pAb at 1:500 dilutionLane1:Hela cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate