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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00007369-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007369-M03, RRID:AB_1716832
- Product name
- UMOD monoclonal antibody (M03), clone 3F10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant UMOD.
- Antigen sequence
DTSEARWCSECHSNATCTEDEAVTTCTCQEGFTGD
GLTCVDLDECAIPGAHNCSANSSCVNTPGSFSCVC
PEGFRLSPGLGCTDVDECAEPGLSHC- Isotype
- IgG
- Antibody clone number
- 3F10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Mass spectrometric study of stone matrix proteins of human bladder stones.
Proteomic study of renal uric acid stone.
Jou YC, Tsai YS, Fang CY, Chen SY, Chen FH, Huang CH, Li YH, Shen CH
Urology 2013 Aug;82(2):295-300
Urology 2013 Aug;82(2):295-300
Proteomic study of renal uric acid stone.
Jou YC, Fang CY, Chen SY, Chen FH, Cheng MC, Shen CH, Liao LW, Tsai YS
Urology 2012 Aug;80(2):260-6
Urology 2012 Aug;80(2):260-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of UMOD expression in transfected 293T cell line by UMOD monoclonal antibody (M03), clone 3F10.Lane 1: UMOD transfected lysate (Predicted MW: 66.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged UMOD is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of UMOD transfected lysate using anti-UMOD monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with UMOD MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
