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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00007369-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007369-M03, RRID:AB_1724999
- Product name
- Mouse Monoclonal Uromodulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Reacts with uromodulin.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Mass spectrometric study of stone matrix proteins of human bladder stones.
Proteomic study of renal uric acid stone.
Jou YC, Tsai YS, Fang CY, Chen SY, Chen FH, Huang CH, Li YH, Shen CH
Urology 2013 Aug;82(2):295-300
Urology 2013 Aug;82(2):295-300
Proteomic study of renal uric acid stone.
Jou YC, Fang CY, Chen SY, Chen FH, Cheng MC, Shen CH, Liao LW, Tsai YS
Urology 2012 Aug;80(2):260-6
Urology 2012 Aug;80(2):260-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Uromodulin Antibody (3F10) [H00007369-M03] - Analysis of UMOD expression in transfected 293T cell line by UMOD monoclonal antibody (M03), clone 3F10. Lane 1: UMOD transfected lysate (Predicted MW: 66.7 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Uromodulin Antibody (3F10) [H00007369-M03] - Analysis of UMOD transfected lysate using anti-UMOD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with UMOD rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Uromodulin Antibody (3F10) [H00007369-M03] - Detection limit for recombinant GST tagged UMOD is 0.3 ng/ml as a capture antibody.
